Compositions for eliciting an immune response against...

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Bacterium or component thereof or substance produced by said...

Reexamination Certificate

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C424S234100, C424S184100, C514S04400A, C536S023700

Reexamination Certificate

active

07858102

ABSTRACT:
The invention provides compositions and method for stimulating an immunological response againstMycobacterium aviumsubspeciesparatuberculosis(MPT). The compositions comprise at least five recombinant immunogenic components. The immunogenic components can be MPT antigens or DNA polynucleotides encoding MPT antigens, or combinations thereof. MPT antigens used in the invention include MPT 85A, 85B, 85C, 35 kDa, super oxide dismutase (SOD), MptC, MptD and ESAT-6 protein. The method comprises administering the composition to an animal in an amount effective to stimulate an immunological response against MPT bacteria. The method is of benefit to any animal susceptible to MPT infection, but is particularly beneficial for ruminants.

REFERENCES:
patent: WO 03/076898 (2003-09-01), None
Uzonna et al.; Efficacy of commercial and field-strainMycobacterium paratuberculosisvaccinations with recombinant IL-12 in a bovine experimental infection model; Vaccine, 2003, vol. 21; pp. 3101-3109.
Mullerad et al.; The immunogenicity ofMycobacterium paratuberculosis85B antigen; Med. Microbiol. Immunol., 2002, vol. 190; pp. 179-187.
Shin et al.; Comparative antibody response of five recombinant antigens in related to bacterial shedding levels and development of serological diagnosis based on 35 kDa antigen forMycobacterium aviumsubsp.paratuberculosis; J. Vet. Sci., 2004, vol. 5, No. 2; pp. 111-117.
Dheenadhayalan, et al.; Cloning and Characterization of the Genes Coding for Antigen 85A, 85B and 85C ofMycobacterium aviumsubsp.paratuberculosis; DNA Sequence, 2002, vol. 13, No. 5; pp. 287-294.
Velaz-Faircloth, et al.; Protection againstMycobacterium aviumby DNA Vaccines Expressing Mycobacterial Antigens as Fusion Proteins with Green Fluorescent Protein; Infection and Immunity, Aug. 1999, vol. 67, No. 8; pp. 4243-4250; XP 002491854.
Sechi et al., Immunization with DNA vaccines encoding different mycobacterial antigens elicits a Th1 type immune response in lambs and protects againstMycobacterium aviumsubspeciesparatuberculosisinfection; (Abstract) Vaccine. Jan. 16, 2006;24(3):229-35.
Shin et al.; In Vitro Cellular Immune Responses to Recombinant Antigens ofMycobacterium aviumsubsp.paratuberculosis; Infection and Immunity, Aug. 2005, vol. 73, No. 8; pp. 5074-5085.
Körmendy;Paratuberculosisvaccine in a large dairy herd; Acta Vet Hung. 1992, vol. 40, No. 3; 1 page.

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