Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1997-08-01
1999-04-06
Hendricks, Keith D.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C12N 902
Patent
active
058916962
ABSTRACT:
A storable biotransformation mixture that can oxidize a drug, comprising a cytochrome P450 enzyme, an electron donor; and, a buffer solution. In a preferred embodiment the mixture also contains liposomes, cytochrome b.sub.5 and NADPH-P450 reductase as the electron donor. The mixture is storable at a temperature less than 8.degree. C. to for at least two weeks and is able to oxidize a drug after storage.
REFERENCES:
Eberhart, Delmont C. et al., "Cytochrome P450 IIIA1 (P450p) Requires Cytochrome b.sub.5 and Phospholipid with Unsaturated Fatty Acids." Archives of Biochemistry and Biophysics 1991; 291; No. 2; 231-240.
Gillam, Elizabeth M. J. et al., "Expression of Modified Human Cytochrome P450 3A4 in Escherichia coli and Purification and Reconstitution of the Enzyme." Archives of Biochemistry and Biophysics 1993; 305; No. 1; 123-131.
Gillam, Elizabeth M. J. et al., "Expression of Cytochrome P450 3A5 in Escherichia coli: Effects of 5' Modification, Purification, Spectral Characterization, Reconstruction Conditions, and Catalytic Activities." Archives of Biochemistry and Biophysics 1995; 317; No. 2; 374-384.
Gorski, J. Christopher et al., "Regioselective Biotransformation of Midazolam by Members of the Human Cytochrome P450 3A (CYP3A) Subfamily." Biochemical Pharmacology 1994; 47; No. 9; 1643-1653.
Guengerich, F. Peter et al., "Characterization of Rat and Human Liver Microsomal Cytochrome P-450 Forms Involved in Nifedipine Oxidation, a Prototype for Genetic Polymorphism in Oxidative Drug Metabolism." The Journal of Biological Chemistry 1986; 261; No. 11; 5051-5060.
Holmans, Priscilla L. et al., "The High-Level Expression in Eschericha coli of the Membrane-Bound Form of Human and Rat Cytochrome b.sub.5 and Studies on Their Mechanism of Function." Archives of Biochemistry and Biophysics 1994; 312; No. 2; 554-565.
Imaoka, Susumu et al., "Role of Phospholipids in Reconstituted Cytochrome P450 3A Form and Mechanism of Their Activation of Catalytic Activity." Biochemistry 1992; 31; 6063-6069.
Ingelman-Sundberg, Magnus et al., "High Rates of Substrate Hydroxylation by Human Cytochrome P450 3A4 in Reconstituted Membranous Vesicles: Influence of Membrane Charge." Biochemical and Biophysical Research Communications 1996; 221; 318-322.
Lee, Caroline A. et al., "CYP3A4 Expressed by Insect Cells Infected with a Recombinant Baculovirus Containing Both CYP3A4 and Human NADPH-Cytochrome P450 Reductase Is Catalytically Similar to Human Liver Microsomal CYP3A4." Archives of Biochemistry and Biophysics 1995; 319; No. 1; 157-167.
Muller-Enoch, Dieter et al., "Interaction of Liver Microsomal Cytochrome P-450 and NADPH-Cytochrome P-450 Reductase in the Presence and Absence of Lipid." The Journal of Biological Chemistry 1984; 259; No. 13; 8174-8182.
Nash, T., "The Colorimetric Estimation of Formaldehyde by Means of the Hantzsch Reaction." Air Hygiene Laboratory, Public Health Laboratory Service 1953; 55; 416-421.
Nelson, David R. et al., "P450 Superfamily: Update on New Sequences, Gene Mapping, Accession Numbers Nomenclature." Pharmacogenetics 1996; 6; 1-42.
Parkinson, Andrew, "Biotransformation of Xenobiotics,", (date N.A.).
Penman, B.W. et al., "Characterization of a Human Cell Line Expressing High Levels of cDNA-derived CYP2D6." Pharmacogenetics 1993; 3; 28-39.
Shaw, Peter M. et al., "Purification and Characterization of an Anticonvulsant-Induced Human Cytochrome P-450 Catalysing Cyclosporin Metabolism." Biochem. J. 1989; 263; 653-663.
Shaw, Peter M. et al., "Reconstitution Premixes for Assays Using Purified Recombinant Human Cytochrome P450, NADPH-Cytochrome P450 Reductase, and Cytochrome b.sub.5 -" Archives of Biochemistry and Biophysics 1997; 348; No. 1; 107-115.
Shen, Anna L. et al., "Structural Analysis of the FMN Binding Domain of NADPH-Cytochrome P-450 Oxidoreductase by Site-directed Mutagenesis." J. Biological Chemistry 1989; 264; No. 13; 7584-7589.
Shephard, Elizabeth A. et al., "Quantification of Cytochrome P450 Reductase Gene Expression in Human Tissues." Archives of Biochemistry and Biophysics 1992; 294; No. 1; 168-172.
Ueng, Yune-Fang et al., "Cooperativity in Oxidations Catalyzed by Cytochrome P450 3A4." Biochemistry 1997; 36; 370-381.
Werringloer, Jurgen, "Assay of Formaldehyde Generated During Microsomal Oxidation Reactions." Microsomal Electron Transport and Cyt P-450 297-302 (date N.A.).
Yamazaki, Hiroshi et al., "Roles of Divalent Metal Ions in Oxidations Catalyzed by Recombinant Cytochrome P450 3A4 and Replacement of NADPH-Cytochrome P450 Reductase with Other Flavoproteins, Ferredoxin, and Oxygen Surrogates." Biochemistry 1995; 34; 8380-8389.
Lowery Robert G.
Shaw Peter M.
Thompson David V.
Hendricks Keith D.
Johnson Mark K.
PanVera Corporation
LandOfFree
Compositions for cytochrome P450 biotransformation reactions does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Compositions for cytochrome P450 biotransformation reactions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Compositions for cytochrome P450 biotransformation reactions will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1370486