Compositions containing enzymes bound to cationic copolymers

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process...

Reexamination Certificate

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C435S176000, C435S177000, C435S180000

Reexamination Certificate

active

10472717

ABSTRACT:
Disclosed are a method of adhering active enzymes to an inert support, the product produced thereby, and a method of using the enzyme-coated support in enzyme-catalyzed reactions such as the glucose isomerase-catalyzed conversion of glucose to fructose. The method includes the steps of coating an inert support with a cationic copolymer, preferably a polyamine, and most preferably a di-C1-C6-alkylamino-epichlorohydrin copolymer, and then adhering enzyme to the coated support in the absence of any intervening cross-linking agent.

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patent: 4438196 (1984-03-01), Lantero, Jr.
patent: 4918016 (1990-04-01), Leuba et al.
patent: 5166064 (1992-11-01), Usui et al.
patent: 5314810 (1994-05-01), Kono et al.
patent: 5885609 (1999-03-01), Amiji
International Search Report for PCT/US02/11963.

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