Compositions containing cultured mitotic human stem cells

Details Compositions containing cultured mitotic human stem cells Compositions containing cultured mitotic human stem cells

1994-12-01

1997-09-23

Ziska, Suzanne E.

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

424 933, 4352402, C12N 500, A01N 6300

Patent

active

056701470

ABSTRACT:
Methods, including culture media conditions, which provide for ex vivo human stem cell division and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells are disclosed. The methods rely on culturing human stem cells and/or human hematopoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally growth factors are added to the culture medium.

REFERENCES:
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