Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of culturing cells in suspension
Reexamination Certificate
2007-01-09
2007-01-09
O'Hara, Eileen B. (Department: 1646)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of culturing cells in suspension
C435S325000, C435S366000, C435S371000, C435S395000, C424S198100, C514S002600
Reexamination Certificate
active
10165860
ABSTRACT:
The invention relates to compositions comprising cell culture medium conditioned by cells grown in three-dimensional culture. The cells used to condition the medium may be genetically modified to alter the concentration of growth factors and antioxidants in the medium. The conditioned cell medium (conditioned medium) may be used for at least one of cosmetic applications, cosmeceutical applications, and pharmaceutical applications, among other things. The invention also relates to proteins comprising a heterologous sequence that enhances cell penetration. The invention also relates to cells comprising DNA encoding such proteins. Methods for preparing the inventive compounds are also provided.
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Pinney, Emmett et al., “Human Three-Dimensional Fibroblast Cultures Express Angionenic Activity,” Journal of Cellular Physiology, vol. 183, No. 1, Apr. 2000, pp. 74-82.
Pinney, Emmett et al., “Wound Healing Potential of Dermagraft® Conditioned Medium,” Journal of Investigative Dermatology, vol. 114, No. 4, Apr. 2000, p. 828.
O'Hara Eileen B.
Skin Medica, Inc.
Wilson Sonsini Goodrich & Rosati
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