Compositions and systems for identifying and comparing...

Details Compositions and systems for identifying and comparing... Compositions and systems for identifying and comparing...

2005-10-18

2005-10-18

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091100, C435S091200, C536S023100, C536S024300, C536S024330

Reexamination Certificate

active

06955876

ABSTRACT:
The invention comprises compositions and systems to identify and compare expressed genes in a given in vivo or in vitro RNA sample, as well as the relative difference in mRNA expression between two or more samples, where desired. Furthermore, the invention comprises compositions and systems to identify novel genes. The invention comprises, without limitation, one or more mRNA specific identimers for use in reverse transcription that themselves comprise an oligo-T nucleotide sequence (at the 5′ end) linked to a nucleotide sequence VNx (at the 3′ end) where the V nucleotide immediately adjacent to the oligo-T segment is not a T.

REFERENCES:
patent: 5914229 (1999-06-01), Loewy
patent: 6120996 (2000-09-01), Belyavsky et al.
patent: 6270966 (2001-08-01), Weinstein et al.
patent: WO 99/42610 (1999-08-01), None
patent: WO 00/40757 (2000-07-01), None
Kohroki et al. A Novel Strategy for identifying differential gene expression: An improved method of differential display analysis. Biochemical and Biophysical Research communications, vol. 262, pp. 365-367, Aug. 1999.

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