Compositions and methods utilizing the yeast ZE01 promoter

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S006120, C435S029000, C435S091400, C435S091410, C435S252300, C435S254200, C435S320100, C435S471000, C536S024100, C536S023100

Reexamination Certificate

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07078190

ABSTRACT:
The invention provides novel yeast promoters useful for controlling the expression of homologous and heterologous nucleic acid molecules in yeast cells. The yeast promoters are induced by a fermentable carbon source, such as glucose, or a non-fermentable carbon source, such as ethanol, or both. Therefore, expression of nucleic acid molecules encoding a polypeptide under the control of the novel yeast promoters may be regulated by varying the level of a fermentable carbon source, or a non-fermentable carbon source, or both.

REFERENCES:
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The Nucleotide Sequence of . . . ; M. Johnson et al., Nature, vol. 387, Supp. pp. 87-90 (1997).
Yeast Sequencing Reports, Sequence Analysis of a 44kb DNA . . . , M. Vandenbol et al., Yeast, vol. 11, pp. 1069-1075 (1995).
Yeast Sequencing Reports, Sequence Analysis of a 37 6 kbp Cosmid . . . , P. Verhasselt et al. Yeast, vol. 13, pp. 241-250 (1997).
Johnston, M., et al.; “Saccharomyces cervisiaechromosome XII cosmid 9354”;Medline; 97313267; B-Door-External, Terminal 4247-1-2.Log.pp. 5-8; Jul. 24, 2000; Aug. 13, 1997 (Rel. 52, Last Updated, Ver. 3).
Vandenbol, M., et al; “S. cerevisiaechromosome XV DNA (44 Kb fragment)”;Medline; 96076631; B-Door-External Terminal 4247-4.Log, pp. 5-6; Jul. 24, 2000; Mar. 24, 1997 (Rel. 51, Last Updated, Ver. 7).
Hunt, S., et al; “S. cerevisiaechromosome XIII cosmid 9920”; Unpublished; B-Door-External Terminal 4247-2.Log, pp. 3-5; Jul. 24, 2000; Submitted Mar. 10, 1995 to the EMBL/GenBank/DDBJ databases.
Hunt, S., et al; “S. cervisiaechromosome XIII cosmid 9718”; Unpublished; B-Door-External Terminal 4247-3.Log; pp. 3-5; Jul. 24, 2000; Submitted May 19, 1995 to the EMBL/GenBank/DDBJ databases.

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