Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Conjugate or complex
Patent
1995-09-13
1998-11-17
Feisee, Lila
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Conjugate or complex
4242771, 514 2, 530403, 530417, 436543, A61K 39385, A61K 3900, C07K 100, A23J 100
Patent
active
058372512
ABSTRACT:
The present invention relates to methods and compositions for eliciting an immune response and the prevention and treatment of primary and metastatic neoplastic diseases and infectious diseases. The methods of the invention comprise administering a composition comprising an effective amount of a complex, in which the complex consists essentially of a heat shock protein (hsp) noncovalently bound to an antigenic molecule. "Antigenic molecule" as used herein refers to the peptides with which the hsps are endogenously associated in vivo as well as exogenous antigens/immunogens (i.e., with which the hsps are not complexed in vivo) or antigenic/immunogenic fragments and derivatives thereof. In a preferred embodiment, the complex is autologous to the individual. The effective amounts of the complex are in the range of 10-600 micrograms for complexes comprising hsp70, 50-1000 micrograms for hsp90, and 10-600 micrograms for gp96. The invention also provides a method for measuring tumor rejection in vivo in an individual, preferably a human, comprising measuring the generation by the individual of MHC Class I-restricted CD8+ cytotoxic T lymphocytes specific to the tumor. Methods of purifying hsp70-peptide complexes are also provided.
REFERENCES:
patent: 4690915 (1987-09-01), Rosenberg
patent: 5188964 (1993-02-01), McGuire et al.
patent: 5232833 (1993-08-01), Sanders et al.
patent: 5348945 (1994-09-01), Berberian et al.
Paul et al. Int. Arch. Allergy 47:155-60, 1974.
Puyana et al. Surgery 107:442-448, 1990.
Korver et al. J. Immunol. Meth. 74:241-251, 1984.
Huchet, R. Cellular Immunol. 98:188-199, 1986.
Curtis et al. J. Lab. Clin. Med. 78:61-69, 1971.
Blachere et al., 1993, "Immunization with GP96 heat shock proteins isolated from tumors or influenza virus infected cells elicits MHC-restricted, antigen-specific cytotoxic T lymphocytes against the corresponding cells/antigens", J. Cell Biochem. Suppl. 17D:124 (Abstract NZ 502).
Levinson et al., 1979 "Metal Binding Drugs Induce Synthesis of Four Proteins in Normal Cells", Biol. Trace Element Research 1:15-23.
Martin et al., 1986, "Role of Murine Tumor Models in Cancer Treatment Research", Cancer Res. 46: 2189-2192.
Mule et al., 1984, "Adoptive Immunotherapy of Established Pulmonary Metastases with LAK cells and Recombinant Interleukin-2", Science 225: 1487-1489.
Nieland et al., 1996, "Isolation of an immunodominant viral peptide that is endogenously bound to the stress protein GP96/GRP94", Proc. Natl. Acad. Sci. USA 93: 6135-6139.
Srivastava et al., 1993, "Evidence for peptide-chaperoning by the endoplasmic reticular heat shock protein GP96: Implications for vaccination against cancer and infectious diseases", J. Cell Biochem. Suppl. 17D:94 (Abstract NZ014).
Suto, R. and Srivastava, P.K., 1995, "A Mechanism for the Specific Immunogenicity of Heat Shock Protein-Chaperoned Peptides", Science 269: 1585-1588.
Thomas et al., 1982, "Molecular and Cellular Effects of Heat Shock and Related Treatments of Mammalian Tissue-Culture Cells", Cold Springs Harbor Symp Quant. Biol. 46: 985-996.
Arnold et al., 1995, "Cross-priming of minor histocompatibility antigen-specific cytotoxic T cells upon immunization with the heat shock protein gp96", J. Exp. Med. 182:885-889.
Cole & Ostrand-Rosenburg, 1991, "Rejection of allogeneic tumor is not determined by host responses to MHC class I molecules and is mediated by CD4.sup.- CD8.sup.+ T lymphocytes that are not lytic for the tumor", Cellular Immunol. 134:480-490.
Engman et al., 1990, "Human humoral immunity to hsp70 during Trypanosoma cruzi infection", J. of Immunol. 144:3987-3991.
Estes et al., 1993, "Characterization of an unusual cell type (CD4.sup.+ CD3.sup.-) expanded by helminth infection and related to the parasite stress response", J. of Immunol. 150:1846-1856.
C. Ezzel, 1995, "Cancer `Vaccines`: An idea whose time has come?", J. NIH Res. 7:46-49.
Jindal & Young, 1992, "Vaccinia virus infection induces a stress response that leads to association of Hsp70 with viral proteins", J. of Virol. 66:5357-5362.
Johnson et al., 1989, "The 86-kilodalton antigen from Schistosoma mansoni is a heat-shock protein homologous to yeast HSP-90", Mol. and Biochem. Parasitol. 36:19-28.
Luft et al., 1991, "Immunologic and structural characterization of the dominant 66- to 73-kDa antigens of Borrelia burgdoferi", J. of Immunol. 146:2776-2782.
Srivastava et al., 1994, "Heat shock proteins transfer peptides during antigen processing and CTL priming", Immunogenetics 39:93-98.
Udono & Srivastava, 1994, "Comparison of tumor-specific immunogenicities of stress-induced proteins gp96, hsp90, and hsp70", J. of Immunol. 152:5398-5403.
Udono et al., 1994, "Cellular requirements for tumor-specific immunity elicited by heat shock proteins: Tumor rejection antigen gp96 primes CD8.sup.+ T cells in vivo", PNAS USA 91:3077-3081.
White et al., 1988, "Differential distribution of the adenovirus E1A proteins and colocalization of E1A with the 70-kilodalton cellular heat shock protein in infected cells", J. of Virol. 62:4153-4166.
Aldovini et al. (1992) "The New Vaccines", Technology Review pp. 24-31.
Barrios et al. (1992) "Mycobacterial heat-shock proteins as carrier molecules. II: The use of the 70-kDa mycobacterial heat-shock protein as carrier for conjugated vaccines that can circumvent the need for adjuvants and Bacillus Calmette Guerin priming", Eur. J. Immunol. 22:1365-1372.
Basombrio (1970) "Search for common antigenicities among twenty-five sarcomas induced by methylcholanthrene", The Institute for Cancer Research 30:2458-2462.
Blachere et al. (1993) "Heat Shock Protein Vaccines Against Cancer," Journal of Immunotherapy 14:352-356.
Blachere and Srivastava (1993) "Immunization with GP96 heat shock proteins isolated from tumors or influenza virus infected cells elicits MHC-restricted, antigen-specific cytotoxic T lymphocytes against the corresponding cells", J. Cellular Biochem. Keystone Symposia NZ502, p. 124.
Boon "Toward a genetic analysis of tumor rejection antigens", Advances in Cancer Research 58:177-210.
Cohen (1993) "Cancer Vaccines Get A Shot In The Arm", Science 262:841-843.
Craig (1993) "Chaperones: Helpers Along the Pathways to Protein Folding", Science 260:1902-1904.
Elliott et al. (1990) "Naturally Processed Peptides", Nature 348:195-197.
Falk et al. (1991) "Allele-specific Motifs Revealed by Sequencing of Self-peptides Eluted from MHC Molecules", Nature 351:290-296.
Falk et al. (1990) "Cellular Peptide Composition Governed by Major Histocompatibility Complex Class I Molecules", Nature 348:248-251.
Fedweg and Srivastava "Evidence for biochemical heterogeneity of gp96 heat shock protein/tumor rejecion antigen", Mount Sinai School of Medicine NZ 206, p. 108.
Flynn et al. (1989) "Peptide binding and release by proteins implicated as catalysts of protein assembly", Science 245:385-390.
Flynn et al. (1991) "Peptide-binding Specificity of the Molecular Chaperone BiP", Nature 353:726-730.
Franklin (1993) "Making vaccines fit the cancer", New Scientist 140:17.
Gething et al. (1992) "Protein Folding in the Cell", Nature 355:33-45.
Globerson and Feldman (1964) "Antigenic specificity of 32(6):1229-1242.
Jakob et al. (1993) "Small Heat Shock Proteins Are Molecular Chaperones", J. Biol. Chem. 268:1517-1520.
Jardetzky et al. (1991) "Identification of Self Peptides Bound to Purified HLA-B27", Nature 353:326-329.
Lakey et al (1987) "Identification of a peptide binding protein that plays a role in antigen presentation", Proc. Natl. Acad. Sci. USA 84:1659-1663.
Lanzavecchia (1993) "Identifying Strategies for Immune Intervention", Science 260:937-944.
Levy (1991) "ATP is Required for In Vitro Assembly of MHC Class I Antigens but Not for Transfer of Peptides across the ER Membrane", Cell 67:265-274.
Li and Srivastava (1993) "Tumor rejection antigen gp96/grp94 is an ATPase: Implications for protein folding and antigen presentation", EMBO J. 12(8):3143-3151.
Lindquist and Craig (1988) "The heat-shock proteins", Ann. Rev. Genet. 22:631-677.
Luescher et al. (1991) "Specific Binding of Antigenic Peptides to Cell-associated MHC Clas I Molecules", Nat
Bansal Gee Tha D.
Feisee Lila
Fordham University
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