Chemistry: molecular biology and microbiology – Vector – per se
Patent
1995-01-17
1998-07-14
Grimes, Eric
Chemistry: molecular biology and microbiology
Vector, per se
536 232, C12N 1563
Patent
active
057802963
ABSTRACT:
The invention concerns genes encoding recombinases that can be used to promote homologous recombination in eukaryotic cells and expression vectors that can be used to transiently express recombinases in target cells. One embodiment of the invention encompasses genetically engineered nucleic acids that encode a non-naturally occurring recombinase that causes a greater rate of recombination than does the naturally occurring recombinase. Recombinases from Ustilago maydis, Saccharomyces cerevisiae are specifically included in the invention.
REFERENCES:
patent: 4873191 (1989-10-01), Wagner et al.
patent: 4950599 (1990-08-01), Bertling et al.
patent: 5139941 (1992-08-01), Muzyczka et al.
patent: 5354678 (1994-10-01), Lebkowski et al.
Thomas, "Recombination of DNA Molecules", Prog Nuc Acid Res Mol Biol 5;315 (1966).
Holliday et al., "Altered Recombination Frequencies in Radiation Sensitive Strains of Ustilago", Mutation Res 4:275-288 (1967).
Sande et al., "Phosphorylation of N-Protected Deoxyoligonucleotides by T4 Polynucleotide Kinase", Biochem 12;5056-5062 (1973).
Holliday et al., "Genetic Characterization of rec-1, a Mutant of Ustilago maydis Defective in Repair and recombination", Genet Res 27:413-453 (1976).
van Weezenbeek et al., "Nucleotide Sequence of the Filamentous Bacteriophage M13 DNA Genome Comparison with Phage fd", Gene 11:129 (1980).
Doolittle, "Similar Amino Acid Sequences: Chance or Common Ancestry?", Science 241:149 (1981).
Bianchi et al., "Synapsis and the Formation of Paranemic Joints by E. coli RecA Protein ", Cell 34:931 (1983).
Kmiec and Holloman, "Heteroduplex Formation and Polarity During Strand Transfer Promoted by Ustiligo Rec 1 Protein", Cell 33:857-864 (1983).
Bradley et al., "Formation of Germ-line Chimaeras from Embryo-Derived Teratocarcinoma Cell Lines". Nature 309:255-256.
Kmiec and Holloman, "Synapsis Promoted by Ustiligo Rec 1 Protein", Cell 36:593-598 (1984).
Kmiec and Holloman, "Homologous Pairing of DNA Molecules by Ustiligo Rec 1 Protein Is Promoted by Sequences of Z-DNA", Cell 44:545-554 (1986).
Studier and Moffatt, "Use of Bacteriophage T7 RNA Polymerase to Direct Selective High-level Expression of Cloned Genes", J Mol Biol 189:113-130 (1986).
Thomas and Capecchi, "Site-Directed Mutagenesis by Gene Targeting in Mouse Embryo-Derived Stem Cells", Cell 51:503-512 (1987).
Moerschel et al., "Transformation of Yeast with Synthetic Oligonucleotides", PNAS USA 85:524 (1988).
Pearson et al., "Improved Tools for Biological Sequence Comparison", PNAS USA 85:2444-2448 (1988).
Brinster et al., "Targeted Correction of a Major Histocompatibility Class II E.alpha.Gene by DNA Microinjected into Mouse Eggs", PNAS USA 86:7087-7091 (1989).
Capecchi, "Altering the Genome by Homologous Recombination", Science 244:1288-1292 (1989).
Chisaki and Capecchi, "Regionally Restricted Developmental Defects Results from targeted Disruption of the Mouse Homebox Gene hox-1.5", Nature 350:473-479 (1989).
Ho et al., "Site-directed Mutagenesis by Overlap Extension Using the Polymerase Chain Reaction", Gene 77:51-59 (1989).
Shenoy et al., "Purified Maturation Promoting Factor Phosphorylates pp60.sup.c-src at the Sites Phosphorylated during Fibroblast Mitosis", Cell 57:763-774 (1989).
Tsukuda et al., "Isolation of the REC1 Gene Controlling Recombination in Ustiligo maydis", Gene 85:335-341 (1989).
Bauchwitz and Holloman, "Isolation of the REC2 Gene Controlling Recombination in Ustiligo maydis", Gene 96:285-288 (1990).
Lewin, "Driving the Cell Cycle: M Phase Kinase, Its Partners and Substrates", Cell 61:743-752 (1990).
Pearson, "Rapid and Sensitive Sequence Comparison with FASTP and FASTA", Methods in Enzymology, 183:63-98 (1990).
Fotheringham and Holloman, "Extrachromosomal Recombination Is Deranged in the rec2 Mutant of ustiligo maydis", Genetics 129:1053-1060 (1991).
Hunger, "The t(1;19)(q23;p13) Results in Consistent Fusion of E2A and PB1X1 Coding Sequences in Acute Lymphoblastic Leukemias", Blood 77:687-693 (1991).
Reed, "The Role of p34 Kinases in the G1 to S-Phase Transition", Ann Rev Cell Biol 8:529-561 (1992).
Shenoy et al., "Role of p34.sup.cdc2 -mediated Phosphorylations in Two-step Activation of pp60.sup.c-scr During Mitosis", PNAS USA 89:7237-7241 (1992).
Rao and Radding, "Homologous Recognition Promoted by RecA Protein Via non-Watson-Cricks Bonds Between Identical DNA Strands", PNAS USA 90:6646-6650 (1993).
Crystal et al., "Administration of an Adenovirus Containing the Human CFTR cDNA to the Respiratory Tract of Individuals with Cystic Fibrosis", Nature Genetics 8:42-51 (1994).
Kaplitt et al., "Long-term Gene Expression and Phenotypic Correction Using Adeno-Associated Virus Vectors in Mammalian Brain", Nature Genetics 8:148-154 (1994).
Kmiec and Holloman, "ATP Dependent DNA Renaturation and DNA-dependent ATPase Reactions Catalyzed by the Ustiligo maydis Homologous Pairing Protein", Eur J Bioch 219:865-875 (1994).
Kmiec and Holloman, "DNA Strand Exchange in the Absence of Homologous Pairing", J Biol Chem 269:10163-10168 (1994).
Pines, "Ubiquitin With Everything", Nature 371:742-743 (1994).
Rubin et al., "Structure of REC2, a Recombinatorial Repair Gene of Ustilago maydis, and Its Function in Homologous Recombination between Plasmid and Chromosomal Sequences", Mol Cell Biol 14:6287-6296 (1994).
Holloman William K.
Kmiec Eric B.
Grimes Eric
Hansburg Daniel
Thomas Jefferson University
LandOfFree
Compositions and methods to promote homologous recombination in does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Compositions and methods to promote homologous recombination in , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Compositions and methods to promote homologous recombination in will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1880913