Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Medium – per se – for culture – maintenance – regeneration – etc.
Patent
1995-06-02
1998-05-12
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Medium, per se, for culture, maintenance, regeneration, etc.
435410, 435420, 504118, C12N 500, C12N 502
Patent
active
057504024
ABSTRACT:
The present invention encompasses compositions and methods to reduce or prevent microbial growth in plant tissue culture media, comprising adding a chemical agent comprising methylchloroisothiazolinone, methylisothiazolinone, magnesium chloride and magnesium nitrate to a plant culture medium at a concentration that reduces or prevents microbial contamination of the plant tissue culture medium and that allows substantially normal germination of seeds or substantially normal growth or development of plants, plant organs, plant tissues or plant cells. The chemical agent may further comprise potassium sorbate or sodium benzoate, or both. The present invention further provides a kit for germinating plant seeds or culturing plants, plant organs, plant tissues or plant cells on a plant tissue culture medium comprising the chemical agent.
REFERENCES:
patent: 3122432 (1964-02-01), Biggs
patent: 3523121 (1970-08-01), Lewis et al.
patent: 3761488 (1973-09-01), Lewis et al.
patent: 4105431 (1978-08-01), Lewis et al.
patent: 4243403 (1981-01-01), Lewis et al.
patent: 4252694 (1981-02-01), Lewis et al.
patent: 4265899 (1981-05-01), Lewis et al.
patent: 4279762 (1981-07-01), Lewis et al.
patent: 4454146 (1984-06-01), Borovian
patent: 4499071 (1985-02-01), Borovian
patent: 4540570 (1985-09-01), Borovian
patent: 4555400 (1985-11-01), Borovian
patent: 5028620 (1991-07-01), Hsu
patent: 5100905 (1992-03-01), Hsu
Chapman, 1994, "An effective biocide for in vitro diagnostic reagents and other products", Am Clin Lab Aug. 1994, pp. 13-14.
Francko, 1986, "Studies on Nelumbo lutea (Willd.) Pers. I. Techniques for axenic liquid seed culture", Aquatic Botany 26:113-117.
Gilbert et al., 1991, "The use of antibiotics to eliminate latent bacterial contamination in potato tissue cultures", Ann Appl Biol 119:113-120.
Green, 1993, "Efficacy of biocides on laboratory-generated Legionella biofilms", Lett Appl Microbiol 17:158-161.
Gruvberger et al., 1986, "Demonstration of Kathon.RTM. CG in some commercial products", Contact Dermatitis 15:24-27.
Haack and Warwick, 1993, "Controlling microbial growth in aqueous-based pesticide formulations", in Pesticide Formulations and Application Systems, Devisetty et al. (eds.), pp. 105-115.
Haldeman et al., 1987, "Use of benomyl and rifampicin for in vitro shoot tip culture of Camellia sinensis and C. japonica", Hortscience 22(2):306-307.
Hall, 1988, "Comparative activity of selected food preservatives as citrus postharvest fungicides", Proc Fla State Hort Soc 101:184-187.
Idise and Izuagbe, 1985, "Effect of preservatives and pasteurization on microorganisms isolated from Nigerian bottled palm wine", Microbios Letters 28:117-121.
Kneifel and Leonhardt, 1992, "Testing of different antibiotics against Gram-positive and Gram-negative bacteria from plant tissue culture", Plant Cell, Tissue and Organ Culture 29:139-144.
Macek et al., 1994, "Chemical sterilization of nutrient media for plant cell cultures using diethylpyrocarbonate", Biotechnology Techniques 8(12):885-888.
The Merck Index, 10th Edition, 1983, Entry No. 7555 and No. 8413.
Phillips et al., 1981, "Antibiotics in plant tissue culture: Rifampicin effectively controls bacterial contaminants without affecting the growth of short-term explant cultures of Helianthus tuberosus", Plant Science Letters 21:235-240.
Pollock et al., 1983, "The toxicity of antibiotics to plant cell cultures", Plant Cell Reports 2:36-39.
Ryu and Holt, 1993, "Growth inhibition of Penicillium expansum by several commonly used food ingredients" J Food Protection 56(10)862-867.
Sodeko et al., 1987, "Effect of different preservative treatments on the microbial population of Nigerian orange juice", Microbios 51:133-143.
Guri Assaf Z.
Patel Kishor N.
Lankford , Jr. Leon B.
Plant Cell Technology, Inc.
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