Compositions and methods of using a synthetic Dnase I

Details Compositions and methods of using a synthetic Dnase I Compositions and methods of using a synthetic Dnase I

2006-06-27

2006-06-27

Patterson, Jr., Charles L. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S255500, C435S320100, C536S023200

Reexamination Certificate

active

07067298

ABSTRACT:
Compositions and method for making and using a synthetic bovine DNase I are disclosed. More particularly, the sbDNase I of the present invention is a versatile enzyme that cleaves DNA nonspecifically to release 5′-phosphorylated nucleotides. The sbDNase I molecules of the present invention find particular use in a wide range of molecular biology applications, including: degradation of contaminating DNA after RNA isolation; RNA clean-up prior to, or in conjunction with, RT-PCR after in vitro transcription; identification of protein binding sequences on DNA (DNase I footprinting); prevention of clumping when handling cultured cells; tissue dissociation and creation of fragmented DNA for in vitro recombination reactions.

REFERENCES:
Liao, T-H., et al. (1973) J. Biol. Chem. 248 (4), 1489-1495.

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