Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-10-02
2007-10-02
Whisenant, Ethan (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S023100, C536S024300
Reexamination Certificate
active
10694480
ABSTRACT:
The present invention relates to methods for detecting the presence of ribonuclease enzymes, more specifically to methods that provide for a visual detection assay. The methods entail contacting a test sample suspected of containing ribonuclease activity with a substrate containing a ribonuclease-sensitive internucleotide linkage flanked directly or indirectly by a fluorescence reporter group and a dark quencher, such that if a ribonuclease activity is present in the sample, the ribonuclease-sensitive internucleotide linkage is cleaved and the fluorescence reporter group emits a visually detectable signal. The present invention further provides novel nucleic acid compositions used as substrates for such assays and encompasses kits for performing the methods of the invention.
REFERENCES:
patent: 5792615 (1998-08-01), Arnold et al.
patent: 6150097 (2000-11-01), Tyagi et al.
patent: 6355421 (2002-03-01), Coull et al.
patent: 6444421 (2002-09-01), Chung
patent: 6451535 (2002-09-01), Jenne et al.
patent: 2002/0102571 (2002-08-01), Theaker et al.
patent: 2002/0110823 (2002-08-01), Hogan
patent: WO99/05314 (1999-02-01), None
patent: WO 99/47704 (1999-09-01), None
Diamond et al. Methods in Enzymology 100 : 431-453 (1983).
The Stratagene Catalog , p. 39 (1988).
Behlke Mark Aaron
Devor Eric Jeffrey
Huang Lingyan
Walder Joseph Alan
Integrated DNA Technologies, Inc.
Petravich John A.
Whisenant Ethan
LandOfFree
Compositions and methods for visual ribonuclease detection... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Compositions and methods for visual ribonuclease detection..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Compositions and methods for visual ribonuclease detection... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3899224