Compositions and methods for utilizing mixed substrate...

Details Compositions and methods for utilizing mixed substrate... Compositions and methods for utilizing mixed substrate...

2005-02-08

2005-02-08

Lankford, Jr., Leon B. (Department: 1651)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

C435S004000, C435S021000, C252S700000, C436S172000

Reexamination Certificate

active

06852503

ABSTRACT:
A dual enzyme chemiluminescent substrate formulation useful for the independent or simultaneous analysis of the enzymes is disclosed, the formulation includes, as one substrate component, dihydrophthalazinedione, such as luminol or isoluminol, and a peroxide source and, as the other substrate component, a 1,2-dioxetane. The formulation further includes a polymeric quaternary onium salt as an isolating agent to defeat adverse interactions between the substrate pairs.

REFERENCES:
patent: 4729950 (1988-03-01), Kricka et al.
patent: 5242842 (1993-09-01), Sundrehagen
patent: 5582775 (1996-12-01), Akhavan-Tafti
patent: 5994073 (1999-11-01), Bronstein et al.
patent: 6068979 (2000-05-01), Akhavan-Tafti

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