Compositions and methods for treating conditions related to...

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai

Reexamination Certificate

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C530S324000, C530S326000

Reexamination Certificate

active

07381701

ABSTRACT:
The present invention relates to compositions and methods of use of cupredoxins, and variants, derivatives and structural equivalents of cupredoxins that interfere with the ephrin signaling system in mammalian cells. Specifically, the invention relates to compositions and methods that use cupredoxins, such as azurin, rusticyanin and plastocyanin, and variants, derivatives and structural equivalents thereof, to treat cancer in mammals.

REFERENCES:
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patent: 2003/0105000 (2003-06-01), Pero et al.
patent: WO 02/076380 (2002-10-01), None
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Yamada T, et al.,“Internalization of bacterial redox protein azurin in mammalian cells: entry domain and specificity”,Cell Microbiol.Oct. 2005; 7(10):1418-31.
Yamada T, et al., “Rusticyanin, a bacterial electron transfer protein, causes G1 arrest in J774 and apoptosis in human cancer cells”,Cell CycleSep. 2004; 3(9):1182-7.
Yamada T, et al.,“Regulation of mammalian cell growth and death by bacterial redox proteins: relevance to ecology and cancer therapy”,Cell CycleJun. 2004;3(6):752-5.
Hiraoka Y , et al., “Modulation of mammalian cell growth and death by prokaryotic and eukaryotic cytochrome c”,Proc Natl Acad Sci U S A.Apr. 27, 2004;101(17):6427-32.
Yamada T, et al., “Apoptosis or growth arrest: Modulation of tumor suppressor p53's specificity by bacterial redox protein azurin”,Proc Natl Acad Sci U S A.Apr. 6, 2004; 101(14):4770-5.
Punj V, et al., “Bacterial cupredoxin azurin as an inducer of apoptosis and regression in human breast cancer”,OncogeneMar. 25, 2004;23(13):2367-78.
Chakrabarty AM, “Microorganisms and Cancer: Quest for a Therapy”,J. Bacteriol. 185(9):2683-86.
Punj V, et al., “Bacterial cupredoxin azurin and its interactions with the tumor suppressor protein p53”,Biochem Biophys Res Commun. Dec. 5, 2003;312(1):109-14.
Punj V, et al., “Energy-generating enzymes ofBurkholderia cepaciaand their interactions with macrophages”,J Bacteriol. May 2003;185(10):3167-78.
Goto M, et al., “Induction of apoptosis in macrophages byPseudomonas aeruginosaazurin: tumour-suppressor protein p53 and reactive oxygen species, but not redox activity, as critical elements in cytotoxicity”,Mol Microbiol. Jan. 2003;47(2):549-59.
Yamada T, et al., “The bacterial redox protein azurin induces apoptosis in J774 macrophages through complex formation and stabilization of the tumor suppressor protein p53”,Infect Immun. Dec. 2002;70(12):7054-62.
Yamada T, et al., “Bacterial redox protein azurin, tumor suppressor protein p53, and regression of cancer”,Proc Natl Acad Sci U S A. Oct. 29, 2002;99(22):14098-103. Epub Oct. 22, 2002.
Zaborina O, et al., “P2Z-Independent and P2Z receptor-mediated macrophage killing byPseudomonas aeruginosaisolated from cystic fibrosis patients”,Infect Immun. Oct. 1999;67(10):5231-42.
Zaborina O, et al., “Secreted products of a nonmucoidPseudomonas aeruginosastrain induce two modes of macrophage killing: external-ATP-dependent, P2Z-receptor-mediated necrosis and ATP-independent, caspase-mediated apoptosis”,MicrobiologyOct. 2000; 146 ( Pt 10):2521-30.
Yang D., et al., “Bacterial redox protein azurin induce apoptosis in human osteosarcoma U2OS cells”,Pharmacological Research2005 52(5):413-421.
Apiyo D. and Wittung-Stafshede, P., “Unique complex between bacterial azurin and tumor-suppressor protein p53”,Bioche. Biophys. Res. Comm.2005 332: 965-968.
Ye, Z., et al. “Selective inducement effect of bacterial redox protein azurin on apoptosis of human osteosarcoma cell line U2OS”,Chinese Journal of Cancer2005, 24(3): 298-304.
Yamada et al., “The Bacterial Redox Protein Azurin Induces Apoptosis in J774 Macrophages through Complex Formation and Stabilization of the Tumor Suppressor Protein p53”,Infection and Immunity, vol. 70 (12), Dec. 2002, pp. 7054-7062.
Anonymous: “Plastocyanin precursor” Database EMBL, Online, Nov. 1, 1997, XP002306632 abstract.
Anonymous: “Rusticyanin precursor” Database EMBL, Online, Mar. 1, 1992, XP002306633 abstract.
Anonymous: “Pseudoazurin precursor” Database EMBL, Online, Feb. 1, 1991, XP002306634 abstract.
Yamada T, et al., “Internalization of bacterial redox protein azurin in mammalian cells: entry domain and specificity”,Cell Microbiol.Oct. 2005; 7(10):1418-31.
Yamada T, et al., “Internalization of bacterial redox protein azurin in mammalian cells: entry domain and specificity”,Cell Microbiol. Oct. 2005;7(10):1418-31.
Yamada T, et al., “Rusticyanin, a bacterial electron transfer protein, causes G1 arrest in J774 and apoptosis in human cancer cells”,Cell CycleSep. 2004;3(9):1182-7.
Yamada T, et al., “Regulation of mammalian cell growth and death by bacterial redox proteins: relevance to ecology and cancer therapy”,Cell CycleJun. 2004;3(6):752-5.
Hiraoka Y, et al., “Modulation of mammalian cell growth and death by prokaryotic and eukaryotic cytochrome c”,Proc Natl Acad Sci U S A. Apr. 27, 2004;101(17):6427-32.
Yamada T, et al., “Apoptosis or growth arrest: Modulation of tumor suppressor p53's specificity by bacterial redox protein azurin”,Proc Natl Acad Sci U S A. Apr. 6, 2004;101(14):4770-5.
Punj V, et al., “Bacterial cupredoxin azurin as an inducer of apoptosis and regression in human breast cancer”,OncogeneMar. 25, 2004;23(13):2367-78.
Chakrabarty AM, “Microorganisms and Cancer: Quest for a Therapy”,J. Bacteriol. 185(9):2683-86, May, 2003.
Punj V, et al., “Bacterial cupredoxin azurin and its interactions with the tumor suppressor protein p53”,Biochem Biophys Res Commun. Dec. 5, 2003;312(1):109-14.
Punj V, et al., “Energy-generating enzymes ofBurkholderia cepaciaand their interactions with macrophages”,J Bacteriol. May 2003;185(10):3167-78.
Goto M, et al., “Induction of apoptosis in macrophages byPseudomonas aeruginosaazurin: tumour-suppressor protein p53 and reactive oxygen species, but not redox activity, as critical elements in cytotoxicity”,Mol Microbiol.Jan. 2003;47(2):549-59.
Yamada T, et al., “The bacterial redox protein azurin induces apoptosis in J774 macrophages through complex formation and stabilization of the tumor suppressor protein p53”,Infect Immun.Dec. 2002;70(12):7054-62.
Yamada T, et al., “Bacterial redox protein azurin, tumor suppressor protein p53, and regression of cancer”,Proc Natl Acad Sci U S A. Oct. 29, 2002;99(22):14098-103. Epub Oct. 22, 2002.
Zaborina O, et al., “P2Z-Independent and P2Z receptor-mediated macrophage killing byPseudomonas aeruginosaisolated from cystic fibrosis patients”,Infect Immun.Oct. 1999;67(10):5231-42.
Zaborina O, et al., “Secreted products of a nonmucoidPseudomonas aeruginosastrain induce two modes of macrophage killing: external-ATP-dependent, P2Z-receptor-mediated necrosis and ATP-independent, caspase-mediated apoptosis”,MicrobiologyOct. 2000; 146 ( Pt 10):2521-30.
Yang D., et al., “Bacterial redox protein azurin induce apoptosis in human osteosarcoma U2OS cells”,Pharmacological Research2005 52(5):413-421

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