Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or...
Patent
1996-06-14
1999-03-09
Saucier, Sandy
Chemistry: molecular biology and microbiology
Differentiated tissue or organ other than blood, per se, or...
435 12, 435 13, 435 2, A01N 102
Patent
active
058798757
ABSTRACT:
The present invention provides solutions and methods for preserving biological material that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of univalent oxyanions and of iodide. The solutions comprise a first neutral solute having a molecular weight of at least about 335 and a solubility in water of at least about 0.3M, and a second neutral solute having a molecular weight of less than about 200 and having both hydrophilic and hydrophobic moieties. The inventive solutions preferably contain CaSO.sub.4, together with combinations of anions and cations from the protein-stabilizing ends of the Hofmeister series, such as K.sub.2 SO.sub.4. Solutions with raffinose or trehalose in combination with trimethyl amine oxide or betaine are particularly preferred.
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Ferguson Alexander B.
Wiggins Philippa M.
Biostore New Zealand
Saucier Sandy
Sleath Janet
Speckman Ann W.
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