Compositions and methods for the enrichment and isolation of Cam

Details Compositions and methods for the enrichment and isolation of Cam Compositions and methods for the enrichment and isolation of Cam

1987-04-13

1990-05-08

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving urea or urease

435 34, 435 18, 435 30, 4352521, C12Q 158, C12Q 104, C12Q 134, C12Q 124

Patent

active

049238019

ABSTRACT:
A method of enrichment and isolation of urease producing organisms from a contaminated specimen by first homogenizing the contaminated specimen in water, then introducing the homogenized contaminated specimen into a solution of urea in an acid, wherein some of the organisms are killed by the acidic medium and remaining organisms are protected from acid attack by creating a protective ammonia by breaking down the urea, and plating the remaining organisms onto a medium which contains antibiotics inhibitory to some of the remaining organisms, but not inhibitory to organisms to be isolated.

REFERENCES:
Washington II, J. A., in Laboratory Procedures in Clinical Microbiology, pp. 194-195 (1985).
Goodwin, C. S. et al, CA 103: 192789d, vol. 103, p. 373 (1985).

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