Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase
Patent
1997-04-15
1999-11-30
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Lyase
435 691, 4352523, 435419, 4353201, 435183, 43525411, 800295, 536 232, 536 243, A01H 700, A01H 500, C12N 100, C12N 510
Patent
active
059941146
ABSTRACT:
The taxadiene synthase gene of Pacific yew has been cloned and its nucleic acid and polypeptide sequence is presented. Truncation or removal of the transit peptide increases expression of the cloned taxadiene synthase gene expression in E. coli cells.
REFERENCES:
Bensen et al., "Cloning and Characterization of the Maize An1 Gene," Plant Cell 7:75-84 (1995).
Cane et al., "Pentalenene Synthase. Purification, Molecular Cloning, Sequencing, and High-Level Expression in Escherichia coli of a Terpenoid Cyclase from Streptomyces UC5319," Biochem. 33:5846-5857 (1994).
Cane et al., "Trichodiene Synthase. Identification of Active Site Residues by Site-Directed Mutagenesis," Biochem. 34:2480-2488 (1995).
Chappell, Joseph, "Biochemistry and Molecular Biology of the Isoprenoid Biosynthetic Pathway in Plants," Ann. Rev. Plant Physiol. Plant Mol. Biol. 46:521-547 (1995).
Chen et al., "Isopenyl Diphyosphate Synthases: Protein Sequence Comparisons, a Phylogenetic Tree, and Predictions of Secondary Structure," Protein Sci. 3:600-607 (1994).
Colby et al., "4S-Limonene Synthase from the Oil Glands of Spearmint (Menta spicata)," J. Biol. Chem. 268:23016-23024 (1993).
Croteau et al., in Taxane Anticancer Agents: Basic Science and Current Status, Georg et al. (eds.), pp. 72-80, American Chemical Society, Washington, D.C. (1995).
Facchini et al., "Gene Family for an Elicitor-Induced Sesquiterpene Cyclase in Tobacco," Proc. Natl. Acad. Sci. USA 89:11088-11092 (1992).
Floss et al., in Taxo1: Science and Applications, Suffness (ed.), pp. 191-208, CRC Press, Boca Raton, FL (1995).
Hezari et al., "Purification and Characterization of Taxa-4(5), 11 (12)-diene Synthase from Pacific Yew (Taxus brevifolia) that Catalyzes the First Committed Step of Taxo1 Biosynthesis," Arch. Biochem. Biophys. 322:437-444 (1995).
Hezari et al., "Taxo1 Biosynthesis: An Update," Planta Med. 63:291-295 (1997).
Hezari et al., "Taxo1 Production and Taxadiene Synthase Activity in Taxus canadensis Cell Suspension Cultures," Arch. Biochem. Biophy. 337:185-190 (1997).
Koepp et al., "Cyclization of Geranylgeranyl Disphosphate to Taxa-4(5),11(12)-diene Is the Committed Step of Taxo1 Biosynthesis in Pacific Yew," J. Biol. Chem. 270:8686-8690 (1995).
LaFever et al., "Diterpenoid Resin Acid Biosynthesis in Conifers: Enzymatic Cyclization of Geranylgeranyl Pyrophosphate to Abietadiene, the Precursor of Abietic Acid," Arch. Biochem. Biophys. 313:139-149 (1994).
Lin et al., "Mechanism of Taxadiene Synthase, a Diterpene Cyclase that Catalyzes the First Step of Taxo1 Biosynthesis in Pacific Yew," Biochem. 35:2968-2977 (1996).
Mau et al., "Cloning of Casbene Synthase cDNA: Evidence for Conserved Structural Features Among Terpenoid Cyclases in Plants," Proc. Natl. Acad. Sci. USA 91:8497-8501 (1994).
McGarvey et al., "Terpenoid Metabolism," Plant Cell 7:1015-1026 (1995).
Orth et al., "Characterization of a cDNA Encoding a Manganese Peroxidase from Phanerochaete chrysosporium: Genomic Organization of Lignin and Manganese Peroxidase-Encoding Genes," Gene:161-165 (1994).
Rajaonarivony et al., "Evidence for an Essential Histidine Residue in 4S-Limonene Synthase and Other Terpene Cyclases," Arch. Biochem. Biophy. 299:77-82 (1992).
Rubenstein et al., "Studies on the Biosynthesis of Taxo1: Total Synthesis of Taxa-4(20),11(12)-diene and Taxa-4(5), 11(12) -diene. The First Committed Biosynthetic Intermediate," J. Org. Chem. 60:7215-7223 (1995).
Savage, "Monoterpene Synthases from Gymnosperms and Angiosperms: Stereospecificity and Inactivation by Cysteinyl-and Arginyl-Directed Modifying Reagents," Arch. Biochem. Biophy. 320:257-265 (1995).
Scolnik et al., "A Table of Some Cloned Plant Genes Involved in Isoprenoid Biosynthesis," Plant Mol. Biol. Rep. 14:305-319 (1996).
Sun et al., "The Arabidopsis GA1 Locus Encodes the Cyclase ent-Kaurene Synthetase A of Gibberellin Biosynthesis," The Plant Cell 6:1509-1518 (1994).
Tarshis et al., "Crystal Structure of Recombinant Farnesyl Diphosphate Synthase at 2.6-.ANG. Resolution," Biochem. 33:10871-10877 (1994).
Vogel et al., "Abietadiene Synthase from Grand Fir (Abies grandis)," Biol. Chem. 271:23262-23268 (1996).
West et al., "Regulation of Terpenoid Biosynthesis in Higher Plants," Rec. Adv. Phytochem. 13:163-198 (1979).
West, in Biosynthesis of Isoprenoid Compounds, Porter et al. (eds.), vol. 1, pp. 375-411, Wiley & Sons, New York, NY (1981).
Wildung et al., "A cDNA Clone for Taxadiene Synthase, the Diterpene Cyclase that Catalyzes the Committed Step of Taxo1 Biosynthesis," J. Biol. Chem. 271:9201-9204 (1996).
Croteau Rodney B.
Wildung Mark R.
Bugaisky Gabriele E.
Washington State University Research Foundation
Wax Robert A.
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