Compositions and methods for reproducing and modulating...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se

Reexamination Certificate

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C435S004000, C435S006120, C435S183000, C435S325000, C435S366000, C536S022100, C536S023100, C536S024100

Reexamination Certificate

active

06852531

ABSTRACT:
An in-vitro system in which mammalian messenger RNA decapping occurs is provided, for use in identifying modulators, deficiencies, and other aspects of the regulation of RNA turnover.

REFERENCES:
Ohno et al. A nuclear cap binding protein from HeLa cells. Dec. 1990. Nucleic Acids Research 18(23): 6989-6995.*
Hellmann et al. A polypeptide which reverses cap analogue inhibition of cell-free protein synthesis. Purification and binidng to capped oligonucleotides. Apr. 1982. Journal of Biological Chemistry 257(8): 4056-4062.*
Robyt et al. Biochemical techniques: Theory and Practice. 1987 Brooks/Cole Publishing Company. pp. 263, 264 and 271.*
Rainen et al. Antibodies distinguishing between intact and alkali-hydrolyzed 7-methylguanosine. Nucleic Acids Research 5: 3877-3889, 1978. Abstract only.*
Tucker & Parker,Mechanism and Control of mRNA Decapping in Saccharomyces cerevisiae, Annu. Rev. Biochem.69:571-95 (2000).
Holcik & Liebhaber,Four Highly Stable Eukaryotic mRNAs Assemble 3′Untranslated Region RNA-Protein Complexes Sharing Cis and Trans Components, Proc. Natl. Acad. Sci. U.S.A.94:2410-2414 (1997).
Morley & Hershey,A Fractionated Reticulocyte Lysate Retains High Efficiency for Protein Synthesis, Biochimie.72:259-264 (1990).
Merrick,Translation of Exogenous mRNAs in Reticulocyte Lysates, Meth. Enzymol.101: 606-615 (1983).

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