Compositions and methods for protein structural determinations

Details Compositions and methods for protein structural determinations Compositions and methods for protein structural determinations

1993-02-05

1995-02-28

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 34, 435 35, 4352402, 43524031, 436 86, 436 89, C12Q 104, C12N 500, G01N 3300

Patent

active

053936698

ABSTRACT:
A method for determining three-dimensional structural information of a protein involves producing the protein in a form substantially labeled with .sup.13 C or .sup.15 N or both and subjecting the protein to nuclear magnetic resonance spectroscopic analysis. The isotopically labeled protein is produced by a method which involves producing a substantially labeled microbial protein hydrolysate, subjecting the protein hydrolyzate to cation exchange chromatography to produce a partially purified labeled amino acid mixture, subjecting the partially purified labeled amino acid mixture to anion exchange chromatography to produce a purified labeled amino acid mixture and supplementing the purified labeled amino acid mixture with isotopically labeled cysteine.

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