Compositions and methods for protein isolation

Details Compositions and methods for protein isolation Compositions and methods for protein isolation

2007-07-03

2007-07-03

Wax, Robert A. (Department: 1656)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S007100, C435S069100, C435S252300, C435S254110, C435S320100, C435S325000, C530S350000, C530S413000

Reexamination Certificate

active

10712137

ABSTRACT:
The invention provides for polynucleotides and vectors comprising at least two tag sequences. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for the chimeric proteins encoded by these polynucleotides. The invention also provides for methods of using the polynucleotides of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.

REFERENCES:
patent: 5747334 (1998-05-01), Kay et al.
patent: 5939288 (1999-08-01), Thornburg
patent: 2002/0061513 (2002-05-01), Seraphin et al.
patent: 2002/0102655 (2002-08-01), Laible et al.
patent: 2005/0032173 (2005-02-01), Rojas et al.
patent: 2005/0079201 (2005-04-01), Rathenow et al.
patent: 2005/0118646 (2005-06-01), Boniface et al.
Zheng et al., “A new expression vector for high level protein production, one step purification and direct isotopic labeling of calmodulin-binding peptide fusion proteins,” Gene, vol. 186, polypeptide. 55-60, 1997.
Keefe et al., “One-Step Purification of Recombinant Proteins Using a Nanomolar-Affinity Streptavidin-Binding Peptide, the SBP-Tag,” Prot. Exp. and Purif, vol. 23, pp. 440-446, 2001.
Hinrichsen & Blackshear, (1993)Regulation of Peptide-Calmodulin Complexes by Protein Kinase Cin vivo. PNAS, 90:1585-1589.
Noonan, et al., (2002)A Calmodulin Binding Site . . ., Arch. Biochem & Biophys., 398(1):132-140.
Puig, et al., (2001)The Tandem Affinity Purification(TAP)Method: A General Procedure of Protein Complex Purification. Methods 24:218-229.
Rigaut, et al., (1999)A Generic Protein Purification Method for Protein Complex Characterization and Proteome Exploration. Nature Biotechnology, 17:1030-1032.
Stofko-Hahn, et al., (1992)A Single Step Purification for Recombinant Proteins. FEBS 302(3):274-278.
Terpe, K., (2003)Overview of tag Protein Fusions: from Molecular and Biochemical Fundamentals to Commercial Systems. Appl. Microbiol. Biotechnol., 60:523-533.
Wilson, et al., (2001)The Use of mRNA Display to Select High-Affinity Protein-Binding Peptides. PNAS, 98(7):3750-3755.
PCT International Search Report, PCT/US04/37819, May 19, 2006.

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