Compositions and methods for producing heterologous polypeptides

Details Compositions and methods for producing heterologous polypeptides Compositions and methods for producing heterologous polypeptides

1998-12-15

1999-12-14

Ketter, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

43525423, C12P 2102

Patent

active

060015977

ABSTRACT:
Pichia methanolica cells in which an alcohol oxidase gene has been disrupted are disclosed. The cells may also be deficient in vacuolar protease activity. The cells are useful as hosts in recombinant protein production methods.

REFERENCES:
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Gellissen et al., Trends in Biotechnology 10: 413-417, 1992.
Jong et al., Soc. Indust. Microbiol. News 46: 199-203, 1996.
Rothstein, Methods in Enzymology 101: 202-211, 1983.
Rothstein, Methods in Enzymology 194: 281-301, 1991.
Romanos et al., Yeast 8: 423-488, 1992.

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