Compositions and methods for producing heterologous polypeptides

Details Compositions and methods for producing heterologous polypeptides Compositions and methods for producing heterologous polypeptides

1996-08-26

1999-09-21

Ketter, James

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

4353201, 536 241, C12N 115, C12N 1511, C12N 1581

Patent

active

059553499

ABSTRACT:
DNA constructs, Pichia methanolica cells, and methods for producing polypeptides using the DNA constructs and cells are disclosed. The DNA constructs comprise a transcription promoter of a Pichia methanolica gene, a DNA segment encoding a polypeptide heterologous to P. methanolica, a transcription terminator of a P. methanolica gene, and a selectable marker. P. methanolica cells containing the DNA constructs are used within methods for producing polypeptides heterologous to P. methanolica. DNA molecules comprising transcription promoters of P. methanolica genes are also disclosed.

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