Compositions and methods for plant transformation and...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...

Reexamination Certificate

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C800S288000, C800S293000, C800S320000, C435S412000, C435S424000, C435S430000, C435S430100, C435S431000, C536S023100

Reexamination Certificate

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07102056

ABSTRACT:
Improved compositions and methods for transformation and regeneration of plants from embryogenic callus are disclosed that include, for example: use of an intermediate-incubation medium after callus induction to increase the competence of the transformed cells for regeneration; dim light conditions during early phases of selection; use of green callus tissue as a target for microprojectile bombardment; and media with optimized levels of phytohormones and copper concentrations.

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