Compositions and methods for optimized hybridization using...

Details Compositions and methods for optimized hybridization using... Compositions and methods for optimized hybridization using...

2007-01-16

2007-01-16

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C564S063000

Reexamination Certificate

active

10435090

ABSTRACT:
The invention provides a composition, kit and method for hybridizing a probe and target at a temperature lower than their standard hybridization temperature. The chemical component added to the composition has a formula R(NH2)C═O, where R is amino or alkyl. A method for use of the chemical component and composition is also disclosed.

REFERENCES:
patent: 5604097 (1997-02-01), Brenner
Oliva et al., Fluorescence In Situ Hybridization Method for Co-Localization of mRNA and GFP, BioTechniques, 2001, vol. 31(1).
Shchepinov,M.S., et al. “Steric Factors Influencing Hybridisation of Nucleic Acids To Oligonucleotide Arrays,”Nucleic Acids Research, 1977, vol. 25, No. 6, pp. 1155-1161.
Blanchard, A.P., et al., “High-Density Oligonucleotide Arrays,” Biosensors & Bioelectronics, 1996, vol. 11, No. 6/7, pp. 687-690.
Wetnur, J.G., “Acceleration of DNA Renaturation Rates,” Biopolymers, 1975, vol. 14, pp. 2517-2524.
Hughes, T.R., et al., “Expression Profiling Using Microarrays Fabricated By An Ink-Jet Oligonucleotide Synthesizer,” Nature Biotechnology, Apr. 2001, vol. 19, pp. 342-347.
Lockhart, D.J. et al., “Expression Monitoring By Hybirdization To High Density Oligonucleotide Arrays,” Nature Biotechnology, Dec. 1996, vol. 14, pp. 1675-1680.
Marton, M.J. et al., “Drug Target Validation and Identification of Secondary Drug Target Effects Using DNA Microarrays,” Nature Medicine, Nov. 1998, vol. 4, pp. 1293-1301.
Guo, Z., et al., “Direct Fluorescence Analysis of Genetic Polymorphisms By Hybridization With Oligonucleotide Arrays On Glass Supports,” Nucleic Acids Research, 1994, vol. 22, pp. 5456-5465.
DeRisi, J.L.., et al., “Exploring The Metabolic And Genetic Control of Gene Expression On A Genomic Scale,” Science, Oct. 1997, vol. 278, pp. 680-686.
Sambrook, J., et al., “Hybridization To Nitrocellulose Filters Containing Replicas Of Bacterial Colonies,” Molecular Cloning-A Labortory Manual, Second Edition, 1989, pp. 1.101-1.104.
Cantor, C.R., et al., “The Conformation of Biological Macromolecules,” Biophysical Chemistry, Part 1, 1980, pp. 176-184.
Kourisky et al., “DNA-RNA Hybridization at Low Temperature In The Presence of Urea, Biochemical and Biophysical Research,” 1970, vol. 41(4), p. 1080-1087.

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