Compositions and methods for nucleic acid delivery to the lung

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai

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424450, 424493, 435458, A61K 4800, A61K 9127, A61K 916, A61K 3170

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active

059943149

ABSTRACT:
A dry powder composition comprises insoluble nucleic acid constructs dispersed within with a hydrophilic excipient material, where the powder particles have an average size in the range from 0.5 .mu.m to 50 .mu.m. Nucleic acid constructs may comprise bare nucleic acid molecules, viral vectors, or vesicle structures. The hydrophilic excipient material will be selected to stabilize the nucleic acid molecules in the constructs, enhance dispersion of the nucleic acid in dry powder aerosols, and enhance wetting of the nucleic acid constructs as they are delivered to moist target locations within the body.

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M. A. Rosenfeld et al. "Adenovirus-Mediated Transfer of a Recombinant .alpha.1-Antitrypsin Gene to the Lung Epithelium in Vivo," Science, (1991) vol. 252:431-434.
S. L. Underwood et al. "A Novel Technique for the Administration of Bronchodilator Drugs Formulated as Dry Powders to the Anaesthetized Guinea Pig," Journal of Pharmacological Methods, (1991) vol. 26:203-210.
R. Stribling et al. "The Mouse as a Model for Cationic Liposome-Based Aerosolized Gene Delivery," Journal of Biopharmaceutical Sciences, (1992) 3(1/2), 255-263.
H. Gershon et al. "Mode of Formation and Structural Features of DNA-Cationic Liposome Complexes Used for Transfection," Biochemistry (1993) vol. 32:7143-7151.
Brown, "Gene Therapy `Oversold` By Researchers, Journalists", The Washington Post, pp. A1, A22, Dec. 8, 1995.

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