Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-02-07
1999-05-25
Arthur, Lisa
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, C12Q 168, C12P 1934
Patent
active
059069180
ABSTRACT:
A method is provided for detecting and quantitating a DNA base modification in a sample of DNA, wherein the method involves an endonuclease digestion step, a labeling step, and a chromatographic separation step. Utilized in the method are compositions including a carrier for the DNA base modification, an internal standard, and a carrier for the internal standard. Also provided are methods for synthesizing the compositions, and kits containing the compositions.
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patent: 5552285 (1996-09-01), Frenkel
"The Photocreactivity of Pyrimidine-Purine Sequences in Some Deoxydinucleoside Monophosphates and Alternating DNA Copolymers," Kumar, S and Davies, R.J.H. Photochemistry and Photobiology vol. 45 No. 5 pp. 571-579, May 1987.
Weinfeld et al. "Influence of nucleic acid base aromaticity on substrate reactivity with enzymes acting on single stranded DNA", Nucleic Acids Res. vol. 21, pp. 621-626, 1993.
Maccubbin et al. "32-P Postlabeling assay for free radical-induced DNA damage:the formamido remnant of thymine", Free Rad. Res. Comm. vol. 18, pp. 17-28, 1993.
Itakura et al. "Chemical Synthesis and Sequence Studies of Deoxyribooligonucleotides which Constitute the Duplex Sequence of the Lactose Operator of Escherichia coli", J. of Biol. Chem. vol. 259, pp. 4592-4600, 1976.
Ide et al. "Thymine glycols and urea residues in M13 DNA constitute replicative blocks in vitro", Nucleic Acids Research, vol. 13, pp. 8035-8052, 1985.
Maccubbin et al., P-Postlabeling Assay for Free Radical-Induced DNA Damage: The Formamido Remnant of Thymine, Sep. 13, 1992, "Free Rad. Res. Comms.", vol. 18, No. 1, pp. 17-28.
Box Harold C.
Budzinski Edwin E.
Iijima Herbert
Maccubbin Alexander E.
Arthur Lisa
Health Research , Inc.
Souaya Jehanne
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