Compositions and methods for manipulating carbon flux in cells

Details Compositions and methods for manipulating carbon flux in cells Compositions and methods for manipulating carbon flux in cells

2006-01-17

2008-10-14

Prouty, Rebecca E. (Department: 1652)

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C435S091100, C435S139000, C435S145000, C435S254200, C435S471000, C435S483000, C435S069100, C435S252320, C435S252330, C435S252340, C435S252350, C435S254210, C435S254230, C435S254900, C536S023200, C536S024100

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07435168

ABSTRACT:
Nucleotide sequences and genetic constructs that can be used to regulate genes encoding enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as aR. oryzaecell, are provided. Methods of manipulating carbon flux in a cell also are provided.

REFERENCES:
Caplen et al., Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. PNAS., 2001, vol. 98 (17): 9742-9747.
Persengiev et al., Nonspecific, concentration-dependent stimulation and represion of mammalian gene expression by small interfering RNAs (siRNAs). RNA, 2004, vol. 10: 12-18.
Brummelkamp et al., A system for stable expression of short interfering RNAs in mammalian cells. Science, 2002, vol. 296: 550-553.

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