Compositions and methods for inhibiting dimerization of primers

Details Compositions and methods for inhibiting dimerization of primers Compositions and methods for inhibiting dimerization of primers

1994-10-20

1996-10-15

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 422102, C12P 1934

Patent

active

055653391

ABSTRACT:
Improvements to the polymerase chain reaction (PCR), a process for in vitro enzymatic amplification of specific nucleic acid sequences, can be achieved by changing the way that PCR reagents are mixed and the enzymatic reaction is started and by the replacement of mineral oil, commonly used as a vapor barrier to minimize solvent evaporation, by a grease or wax. The use of such mixtures allows for the delay of reagent mixing until the first heating step of a PCR amplification, thereby reducing the enzymatic generation of nonspecific products which occurs when a complete mixture of PCR reagents, with or without test sample, stands at room temperature or below. These mixtures increase the shelf-life of PCR reagents and increase protection of the laboratory environment against contamination by PCR product.

REFERENCES:
patent: 4251506 (1981-02-01), Laby
patent: 4522786 (1985-06-01), Ebersole
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5314809 (1994-05-01), Erlich et al.
Li et al., PNAS 87, 4580-4594 (1990).
Chou et al., Nucleic Acids Res. 20(7), 1717-1723 (1992).
D'Aquila et al., Nucleic Acids Res. 19(13),3749 (1991).
Handyside et al., Nature 344, 768-770 (1990).
Tomer et al., Soil Sci. Soc. Am. J. 53(1), 305-308 (1989).
Ward et al., 1989, Nature 341:544-546.
Newton et al., 1988, Nuc. Acids Res. 16(17):8233-8243.
Newton et al., 1989, Nuc. Acids Res. 17(7):2503-2516.
Shibata et al., 1988, J. Exp. Med. 167:225-230.
Kiyabu et al., 1989, Am J. Surg. Pathol. 13(3):221-224.

Affiliated with

Also associated with

Rating

Compositions and methods for inhibiting dimerization of primers does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Compositions and methods for inhibiting dimerization of primers , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Compositions and methods for inhibiting dimerization of primers will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1245158