Compositions and methods for identifying agents that alter...

Details Compositions and methods for identifying agents that alter... Compositions and methods for identifying agents that alter...

2007-11-12

2009-08-04

Ton, Thaian N (Department: 1632)

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

C435S320100, C435S325000, C435S354000

Reexamination Certificate

active

07569221

ABSTRACT:
Provided are compositions and methods for identifying agents that can modulate expression of the human survivin gene. The compositions include eukaryotic cells that contain a human survivin promoter sequence operably linked to a reporter gene. The method comprises determining whether a test agent can modulate transcription from a human survivin promoter sequence by adding a test agent to eukaryotic containing a human survivin promoter sequence operably linked to a reporter gene, measuring expression of the reporter gene and comparing expression of the reporter gene from to a control, wherein an increase or decrease of expression of the reporter gene relative to the control is indicative that the test agent can modulate transcription from a human survivin promoter. The method also comprises the use of SPlucTg mice for preclinical drug identification, including testing of candidate drug toxicity and efficacy.

REFERENCES:
patent: 2005/0026169 (2005-02-01), Cargill et al.
patent: 2008/0108081 (2008-05-01), Luke et al.
Wu, et al.; Molecular Mechanism of Inhibition of Survivin Transcription by the GC-rich Sequence-selective DNA Binding Antitumor Agent, Hedamycin; The Journal of Biological Chemistry, Issue of Mar. 11, 2005, vol. 280, No. 10; pp. 9745-9751.
Ghadersohi, et al.; Prostate-derived Ets transcription factor (PDEF) downregulates survivin expression and inhibits breast cancer cell growth in vitro and xenograft tumor formation in vivo; Breast Cancer Res Treat (2007), vol. 102; pp. 19-30.

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