Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-10-06
1998-07-14
Ketter, James
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435259, 4353171, C12Q 168, C12N 106
Patent
active
057802300
ABSTRACT:
A composition is provided, which comprises a purified cytoplasmic extract from eggs of Xenopus laevis which is capable of supporting activation of human sperm and complete replication of a human sperm genome. Methods of using the extract to achieve complete replication of the sperm genome are disclosed. A flow cytometric method for quantitatively monitoring replication of human sperm genomes is also disclosed. This method can be used for clinical assessment of sperm quality in a test sample of human sperm from an infertile male donor.
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Li Honghua
Xu Yong-Sheng
Child, Jr. John S.
Coriell Institute for Medical Research
Ketter James
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