Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Reexamination Certificate
2006-04-04
2006-04-04
Shukla, Ram R. (Department: 1633)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
C435S320100
Reexamination Certificate
active
07022519
ABSTRACT:
A method for producing recombinant adeno-associated virus in the absence of contaminating helper virus or wild-type virus involves culturing a mammalian host cell containing an rAd/AAV hybrid virus, an AAV rep sequence and an AAV cap sequence under the control of regulatory sequences directing expression thereof. The rAd/AAV hybrid virus contains a rAAV construct to be packaged into an AAV virion in an backbone containing the adenoviral sequences necessary to express E1a and E1b gene products and to permit replication of the hybrid virus. The method of the invention permits replication of the hybrid virus and production of rAAV virion in this host cell in the absence of a helper virus and obviates a subsequent purification step to purify rAAV from contaminating virus.
REFERENCES:
patent: 5436146 (1995-07-01), Shenk
patent: 5622856 (1997-04-01), Natsoulis
patent: 5658776 (1997-08-01), Flotte
patent: 5658785 (1997-08-01), Johnson
patent: 5681731 (1997-10-01), Lebkowski
patent: 5756283 (1998-05-01), Wilson
patent: 5856152 (1999-01-01), Wilson
patent: 5871982 (1999-02-01), Wilson
patent: 6251677 (2001-06-01), Wilson
patent: 6258595 (2001-07-01), Gao
patent: 6274354 (2001-08-01), Wilson
patent: 6365394 (2002-04-01), Gao
patent: 6387368 (2002-05-01), Wilson
patent: 6475769 (2002-11-01), Wilson et al.
patent: 6485966 (2002-11-01), Gao et al.
patent: WO95/06743 (1995-03-01), None
patent: WO95/34670 (1995-12-01), None
patent: WO96/13598 (1996-05-01), None
patent: WO96/14061 (1996-05-01), None
patent: WO96/39530 (1996-12-01), None
patent: WO96/40240 (1996-12-01), None
patent: WO97/12050 (1997-04-01), None
patent: WO97/17458 (1997-05-01), None
patent: WO97/26337 (1997-07-01), None
patent: WO98/09657 (1998-03-01), None
patent: WO98/10086 (1998-03-01), None
patent: WO98/10088 (1999-03-01), None
patent: WO99/14354 (1999-03-01), None
patent: WO99/15685 (1999-04-01), None
B. Carter, in “Handbook of Parvoviruses”, ed. P. Tijsser, CRC Press, pp. 155-168 (1990).
R. Kotin, “Prospects for the Use of Adeno-Associated Virus as a Vector for Human Gene Therapy”, Hum. Gene Ther., 5:793-801 (Jul., 1994).
G. Gao et al, “High Titer Adeno-Associated Viral Vectors from a Rep/Cap Cell Line and Hybrid Shuttle Vector”, Human Gene Therapy, 9(16):2353-2362 (Nov. 1, 1998).
D. Coovert et al, “Gene Therapy for Muscle Diseases”, Current Opinion in Neurology, 7(5):463-470 (Oct. 1, 1994).
H. Berendsen, “A Glimpse of the Holy Grail?”, Science, 282:642-643 (Oct. 23, 1998).
Fisher et al, “Recombinant Adeno-Associated Virus for Muscle Directed Gene Therapy”, Nature Medicine, 3(3):306-312 (Mar., 1997).
Herzog et al, “Stable Gene Transfer and Expression of Human Blood Coagulation Factor IX After Intramuscular Injection of Recombinant Adeno-Associated Virus”, Proc. Natl. Acad. Sci. USA, 94:5804-5809 (May, 1997).
Kessler et al, “Gene Delivery to Skeletal Muscle Results in Sustained Expression and Systemic Delivery of a Therapeutic Protein”, Proc. Natl. Acad. Sci. USA, 93: 14082-14087 (Nov., 1996).
Samulski et al, “Helper-Free Stocks of Recombinant Adeno-Associated Viruses: Normal Integration does not Require Viral Gene Expression”, J. Virol., 63(9):3822-3828 (Sep., 1989).
Alkhatib et al, “High-Level Eucaryotic in vivo Expression of Biologically Active Measles Virus Hemagglutinin by Using an Adenovirus Type 5 Helper-Free Vector System”, J. Virol., 62(8):2718-2727 (Aug., 1988) (Abstract only).
Gao Guangping
Wilson James M
Howson and Howson
Riggins Patrick S.
Shukla Ram R.
The Trustees of the University of Pennsylvania
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