Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-07-12
2005-07-12
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200
Reexamination Certificate
active
06916619
ABSTRACT:
The subject invention relates to methods for detection of mutations in a PKD gene using DHPLC. The invention includes the following aspects: identification of PKD unique sites; design of PKD-specific primers; amplification of PKD-specific products; and analysis of PCR amplified products by DHPLC. The invention further relates to compositions such as identified unique sites and PKD-specific primers, and kits for performing the methods of the invention.
REFERENCES:
patent: 5891628 (1999-04-01), Reeders et al.
patent: 6031088 (2000-02-01), Somlo et al.
patent: 6071717 (2000-06-01), Klinger et al.
patent: 6228591 (2001-05-01), Somlo et al.
patent: 6485960 (2002-11-01), Harris et al.
patent: 2003/0008288 (2003-01-01), Germino et al.
patent: WO 02/06529 (2002-01-01), None
Phakdeekitcharoen, B. et al., (2001), “Mutation Analysis of the Entire Replicated Portion of PKD1 Using Genomic DNA Samples”,J. Am. Soc. Nephrol, 12:955-963.
Perrichot, R.A. et al., (1999), “DGGE screening of PKD1 gene reveals novel mutations in a large cohort of 146 unrelated patients”,Hum. Genet., 105:231-239.
Thomas, R. et al., (1999), “Identification of Mutations in the Repeated Part of the Autosomal Dominant Polycystic Kidney Disease Type 1 Gene, PKD1, by Long-Range PCR”,Am. J. Hum. Genet., 65:39-49.
Watnick, T. et al., (1999), “Mutation Detection of PKD1 Identifies a Novel Mutation Common to Three Families with Aneurysms and/or Very-Early-Onset Disease”,Am. J. Hum. Genet., 65:1561-1571.
Watnick, T.J. et al., (1998), “Somatic Mutation in Individual Liver Cysts Supports a Two-Hit Model of Cystogenesis in Autosomal Dominant Polycystic Kidney Disease”,Molecular Cell, 2:247-251.
Roelfsema, J.H. et al., (1997), “Mutation Detection in the Repeated Part of the PKD1 Gene”,Am. J. Hum. Genet., 61:1044-1052.
Watnick, T.J. et al., (1997), “An unusual pattern of mutation in the duplicated portion of PKD1 is revealed by use of a novel strategy for mutation detection”,Human Molecular Genetics, 6(9):1473-1481.
Neophytou, P. et al., (1996), “Detection of a novel nonsense mutation and an intragenic polymorphism in the PKD1 gene of a Cypriot family with autosomal dominant polycystic kidney disease”,Hum. Genet., 98:437-442.
Peral, B. et al., (1996), “Screening the 3′ Region of the Polycystic Kidney Disease 1 (PKD1) Gene Reveals Six Novel Mutations”,Am. J. Hum. Genet., 58:86-96.
Turco, A.E. et al., (1995), “A novel nonsense mutation in the PKD1 gene (C3817T) is associated with autosomal dominant polycystic kidney disease (ADPKD) in a large three-generation Italian family”,Human Molecular Genetics, 4(8):1331-1335.
Ward, C.J. et al., (1995), “Homo sapienspolycystic kidney disease-associated protein (PKD1) gene, complete eds”, Database EMBL Online, Database Accession No. L39891:1-20.
International Search Report of International Application No. PCT/US01/22035.
Rossetti, et al.,Mutation Analysis of the Entire PKD1 Gene: Genetic and Diagnostic Implications, 2000, Am. J. Hum. Genet., 68:46-63.
Underhill, et al.,Detection of Numerous Y Chromosome Biallelic Polymorphisms by Denaturing High-Performance Liquid Chromatography, 1997, Genome Research, 7:996-1005.
Liu, et al.,Denaturing High Performance Liquid Chromatograph(DHPLC)Used in the Detection of Germline and Somatic Mutations, 1998, Nucleic Acids Research, vol. 26, No. 6, 1396-1400.
Allen Susan Kimberly
Curran John A.
Flynn Kerry Ellen
Garcés Jorge A.
Hennigan Aidan Noel
Athena Diagnostics, Inc.
Horlick Kenneth R.
Palmer & Dodge LLP
Williams Kathleen M.
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