Compositions and methods for differentiating among shigella...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S024300

Reexamination Certificate

active

07888488

ABSTRACT:
The present invention relates to detecting and distinguishing among bacteria that belong to the speciesShigella, includingShigella boydii, Shigella dysenteriae, Shigella flexneri, andShigella sonnei; and detecting and distinguishingShigellabacteria fromEscherichia coli. In particular, the invention includes provision of species-identifying and genus-identifying nucleotides of 16s rRNA or 16s rDNA from the above named species. Nucleic acid probe molecules capable of differentiating among these species and genera by hybridization, along with methods for their use for specific detection in clinical samples, food samples, environmental samples, and the like are also provided.

REFERENCES:
patent: 4724205 (1988-02-01), Karlsson et al.
patent: 4992364 (1991-02-01), Sansonetti et al.
patent: 5041372 (1991-08-01), Lampel et al.
patent: 5084565 (1992-01-01), Parodos et al.
patent: 5089386 (1992-02-01), Stackebrandt et al.
patent: 5189151 (1993-02-01), Baudry et al.
patent: 5217862 (1993-06-01), Barns et al.
patent: 5374718 (1994-12-01), Hammond
patent: 5376528 (1994-12-01), King et al.
patent: 5474796 (1995-12-01), Brennan
patent: 5541308 (1996-07-01), Hogan
patent: 5601984 (1997-02-01), Kohne
patent: 5714321 (1998-02-01), Hogan
Accession No. X80724, Mar. 1996.
Accession No. X80723, Mar. 1996.
Accession No. X80726, Mar. 1996.
Dyson, NJ. Essential Molecular Biology vol. II: A Practical Approach, chapter 5, pp. 111-156, Brown, T.A. ed. Oxford University Press, Oxford, 1992.
Accession No. A14565, Genbank, Sep. 29, 1994.
Accession No. J01695, Genbank, Apr. 15, 1994.
Accession No. U18997, genbank, Dec. 1994.
Accession No. X80679, Feb. 20, 1996.
Accession No. X80729, Feb. 20, 2996.
Accession No. X80678, Feb. 20, 1996.
Accession No. X80730, Feb. 20, 1996.
Ahern, The Scientist, vol. 9, pp. 1-5.
Anderson, Gene Probes 2: Hybridization strategy, Oxford University Press, New York, 1995; Hames and Higgins eds.
Genbank accession No. X96964, NCBI; Apr. 1996; alignment provided.
Genbank accession No. X80726, NCBI; Mar. 1996; alignment provided.
Cilia et al.; Mol. Biol. Evol. vol. 13, pp. 451-461; Feb. 20, 1996.
Genbank accession No. A14565; NCBI, Sep. 1994, alignment provided.
Dyson, NJ. Essential Molecular Biology, vol. II: A Practical Approach, chapter 5, pp. 1111-1156, Brown, T.A. ed. Oxford University Press, Oxford 1992.
Cilia et al., “Sequence Heterogeneities Among 16S Ribosomal RNA Sequences, and Their Effect on Phylogenetic Analyses at the Species Level”, Mol. Biol. Evol. 13(3):451-461, Feb. 1996.
Faruque et al., “Differentiation ofShigella flexneriStrains by rRNA Gene Restriction Patterns”, J. Clinical Microbiology, Nov. 1992, p. 2996-2999.
Nastasi et al., “Molecular Analysis of Strains ofShigella boydiiIsolated in Northern and Southern Italy”, Res. Microbiol. 1990, 141, 1163-1172.
Search toShigelladetection, Nov. 20, 1996.
Search to “Ribosomal RNA” Nov. 18, 1996.
Search to “Shigella” prior to Feb. 20, 1997.
ECL 3′ Oligolabeling and Detection System Instruction Booklet, pp. 6-9 and 30-31. Prior to Feb. 20, 1997.
Invitrogen Catalog page for pCR™II. Prior to Feb. 20, 1997.
GenBank Database printout, 16s rRNA, Aug. 13, 1996.
GenBank S. sonnei 16s rRNA gene. May 9, 1996.
GenBank S. sonnei 16s rRNA gene. Mar. 29, 1996.
GenBank S. boydii 16s rRNA gene. May 9, 1996.
GenBank S. dysenteriae 16s rRNA gene. May 9, 1996.
GenBank S. dysenteriae 16s rRNA gene. Mar. 29, 1996.
GenBank S. flexneri 16s rRNA gene. May 9, 1996.
GenBank S. flexneri 16s rRNA gene. Mar. 29, 1996.

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