Compositions and methods for detection of active proteases

Details Compositions and methods for detection of active proteases Compositions and methods for detection of active proteases

2006-03-21

2006-03-21

Rao, Manjunath N. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S004000, C435S006120, C435S069100, C435S069700, C435S183000, C435S200000, C435S252100, C435S320100, C435S336000, C435S334000, C435S219000, C536S023200, C536S023400, C530S350000

Reexamination Certificate

active

07015023

ABSTRACT:
A novel assay system is disclosed for detecting the presence or amount of selected active proteases in biological samples. The assay system utilizes a chimeric protease detector protein composed of three domains: (1) a repressor domain, (2) a protease cleavage domain specific for the protease to be assayed, and a reporter domain. The reporter domain is not detectable when linked to the repressor domain, but becomes detectable upon release from the repressor domain by protease-mediated cleavage. Thus, the activity of the selected protein can be determined by measuring the amount of detectable reporter in the sample. Methods and test kits for using the novel assay system in a variety of in vitro and in vivo applications are also disclosed.

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