Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1994-10-05
1997-08-12
Kight, John
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 25, 435 42, 435 46, 435810, 435822, 435829, 435849, 435174, 435177, 435179, 435180, 435325, 435349, 435348, 435419, 435365, 435367, 435358, C12Q 100, C12Q 126, C12N 500
Patent
active
056564228
ABSTRACT:
Methods and compositions are provided for the detection of 2,4-dichlorophenoxyacetic acid (2,4-D) and other phenoxy ether compounds. The phenoxy ether bond of 2,4-D is enzymatically cleaved by 2,4-D .alpha.-ketoglutarate dioxygenase to form 2,4-dichlorophenol, which is assayed by the 4-aminoantipyrine method. The enzyme is supplied in a dried form, preferably immobilized on a solid support, and is stable at room temperature for several months even in a highly impure state, e.g., crude cell extracts or dried cells.
REFERENCES:
Amy et al., "Characterization of Aquatic Bacteria and Cloning of Genes Specifying Partial Degradation of 2,4-Dichlorophenoxyacetic Acid," Appl. Environ. Microbiol. 49:1237-1245 (1985).
Chaudhry et al., "Flavobacterium sp. Which Carries the Genes for Degradation of 2,4-Dichlorophenoxyacetate," J. Bacteriol., 170:3897-3902 (1988).
Danganan et al., "Nucleotide Sequence and Functional Analysis of the Genes Encoding 2,4,5-Trichlorophenoxyacetic Acid Oxygenase in Pseudomonas cepacia AC1100," Appl. Environ. Microbiol., 60:4100-4106 (1994).
Daugherty et al., "Degradation of 2,4-Dichlorophenoxyacetic Acid by Pseudomonas cepacia DB01(pR0101) in a Dual-Substrate Chemostat," Appl. Environ. Microbiol., 60:3261-3267 (1994).
Don et al., "Properties of Six Pesticide Degradation Plasmids Isolated from Alcaligenes paradoxus and Alcaligenes eutrophus," J. Bacteriol., 145:681-686 (1981).
Don et al., "Transposon Mutagenesis and Cloning Analysis of the Pathways for Degradation of 2,4-Dichlorophenoxyacetic Acid and 3-Chlorobenzoate in Alcaligenes eutrophus JMP134(pJP4)," J. Bacteriol., 161:85-90 (1985).
Don et al., "Genetic and Physical Map of the 2,4-Dichlorophenoxyacetic Acid-Degradative Plasmid pJP4," J. Bacteriol., 161:466-468 (1985).
Donnelly et al., "Degradation of Atrazine and 2,4-Dichlorophenoxyacetic Acid by Mycorrhizal Fungi at Three Nitrogen Concentrations In Vitro," Appl. Environ. Microbiol., 59:2642-2647 (1993).
Fukumori et al., "Alcaligenes eutrophus JMP134 `2,4-Dichlorophenoxyacetate Monooxygenase` Is an .alpha.-Ketoglutarate-Dependent Dioxygenase," J. Bacteriol., 175:2083-2086 (1993).
Harker et al., "Phenoxyacetic Acid Degradation by the 2,4-Dichlorophenoxyacetic Acid (TFD) Pathway of Plasmid pJP4: Mapping and Characterization of the TFD Regulatory Gene, tfdR," J. Bacteriol., 171:314-320 (1989).
Hultman, "Microbial Detoxification of Phenoxy Alkanoic Herbicide Containers and Rinseates," M.S. Thesis, University of Idaho, 89 pp. (1992).
Perkins et al., "Organization and Sequence Analysis of the 2,4-Dichlorophenol Hydroxylase and Dichlorocatechol Oxidative Operons of Plasmid pJP4," J. Bacteriol., 172:2351-2359 (1990).
Short et al., "Effects of 2,4-Dichlorophenol, a Metabolite of a Genetically Engineered Bacterium, and 2,4-Dichlorophenoxyacetate on Some Microorganism-Mediated Ecological Processes in Soil," App. Environ. Microbiol., 57:412-418 (1991).
Product literature regarding the 2,4-D RaPID Assay.RTM., Ohmicron, Newton, Pennsylvania, 2pp.
Alexander, "Biodegradation and Bioremediation," Academic Press, p. 46.
Emerson et al., "The Condensation of Aminoantipyrine. II. A New Color Test for Phenolic Compounds; III. Reactions with Hydroxypyridines and Hydroxyquinolines; III. (1). The Synthesis of Methylrubazoic Acid; IV. (1). A Study of a New Color Reaction of Pyrazolons; VI. A Study of The Effect of Excess Base on the Reaction of Amino-Antipyrine with Phenolic Compounds in the Presence of Oxidizing Agents; VII. Hydrolysis in the Presence of Nitrous Acid (1)," J. Org.Chem., 8:417-437; 532-537 (1943).
Kaphammer et al., "Cloning and Characterization of tfdS, the Repressor-Activator Gene of tfdB, from the 2,4-Dichlorophenoxyacetic Acid Catabolic Plasmid pJP4," J. Bacteriol., 172:5856-5862 (1990).
Kaphammer et al., "Regulation of tfdCDEF by tfdR of the 2,4-Dichlorophenoxyacetic Acid Degradtion Plasmid pJP4," J. Bacteriol. 172:2280-2286 (1990).
Karns et al., "Regulation of 2,4,5-Trichlorophenoxyacetic Acid and Chlorophenol Metabolism in Pseudomonas cepacia AC1100," Appl. Environm. Microbiol., 46:1182-1186 (1983).
Krueger et al., "Isolation and Identification of Microorganisms for the Degradation of Dicamba," J. Agr. Food Chem., 37:534-538 ( 1989).
Krueger et al., "Use of Dicamba-Degrading Microorganisms to Protect Dicamba Susceptible Plant Species," J. Agr. Food Chem., 39:1000-1003 (1991).
Lappin et al., "Degradation of the Herbicide Mecoprop [2-(2-Methyl-4-Chlorophenoxy) Propionic Acid] by a Synergistic Microbial Community," Appl. Environ. Microbiol., 49:429-433 (1985).
Perkins et al., "Duplication of a 2,4-Dichlorophenoxyacetic Acid Monooxygenase Gene in Alcaligenes eutrophus JMP134(pJP4)," J. Bacteriol., 170:5669-5672 (1988).
Streber et al., "Analysis, Cloning, and High-Level Expression of 2,4-Dichlorophenoxyacetate Monooxygenase Gene tfdA of Alcaligenes eutrophus JMP134," J. Bacteriol., 169:2950-2955 (1987).
Tett et al., "Biochemistry of Mecoprop Degradation by AlCaligenes denitrificans," Abstract No. Q-85 of the General Meeting, American Society for Microbiology (1994).
Kilbane et al., "Biodegradation of 2,4,5-Trichlorophenoxyacetic Acid by a Pure Culture of Pseudomonas cepacia," Appl. Environ. Microbiol., 44:72-78 (1982).
Pieper et al., "Metabolism of 2,4-Dichlorophenoxyacetic Acid, 4-Chloro-2-Methylphenoxyacetic Acid and 2-Methylphenoxyacetic Acid by Alcaligenes eutrophus JMP 134," Arch. Microbiol., 150:95-102 (1988).
Kozyreva et al., "Metabolism of the Herbicide 2,4-D by a Nocardioides Simplex Strain," Plenum Publishing Corp., pp. 78-85 (1993), Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino. Translated from Mikrobiologiya, vol. 62, No. 1, pp. 110-119, Jan.-Feb., 1993. Original article submitted Jul. 14, 1992.
Tiedje et al., "Enzymatic Cleavage of the Ether Bond of 2,4-Dichlorophenoxyacetate," J. Agr. Food Chem., 17:1080-1084 (1969).
Bradley et al., "Biological Process for the Treatment of Water Contaminated with 2,4-Dichlorophenoxyacetic Acid (2,4-D)," Abstract No. Q-274 of the General Meeting, American Society for Microbiology (1994).
King et al., "Assay for Detection and Enumeration of Genetically Engineered Microorganisms Which is Based on the Activity of a Deregulated 2,4-Dichlorophenoxyacetate Monooxygenase," Appl. Environ. Microbiol., 57:1790-1792 (Jun. 1991).
Crawford Ronald L.
Gu Yongxiang
Knaebel David B.
Korus Roger A.
Idaho Research Foundation Inc.
Kight John
Leary Louise
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