Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2011-08-30
2011-08-30
Whisenant, Ethan C (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S023100, C536S024300
Reexamination Certificate
active
08008017
ABSTRACT:
The present invention describes novel oligonucleotides targeted to nucleic acid sequences derived fromCryptosporidiumorganisms, andCryptosporidium parvumorganisms in particular, which are useful for determining the presence ofCryptosporidiumorganisms in a test sample. The oligonucleotides of the present invention include hybridization assay probes, helper probes and amplification primers. The present invention further describes a novel method for obtaining purified ribonucleic acid from viable oocysts.
REFERENCES:
patent: 4851330 (1989-07-01), Kohne
patent: 5030557 (1991-07-01), Hogan et al.
patent: 5399491 (1995-03-01), Kacian et al.
patent: 5474796 (1995-12-01), Brennan
patent: 5539082 (1996-07-01), Nielsen et al.
patent: 5556774 (1996-09-01), Wiedenmann et al.
patent: 5591434 (1997-01-01), Jenkins et al.
patent: 5595874 (1997-01-01), Hogan et al.
patent: 5690825 (1997-11-01), Parton
patent: 5693472 (1997-12-01), Steele et al.
patent: 5770368 (1998-06-01), De Leon et al.
patent: 5789190 (1998-08-01), Crabb et al.
patent: 5820767 (1998-10-01), Kane et al.
patent: 5840488 (1998-11-01), Hogan
patent: 5925517 (1999-07-01), Tyagi et al.
patent: 6054279 (2000-04-01), Nadeau et al.
patent: 6063604 (2000-05-01), Wick et al.
patent: 6110665 (2000-08-01), Fenger et al.
patent: 6130038 (2000-10-01), Becker et al.
patent: 6146838 (2000-11-01), Williams et al.
patent: 6146855 (2000-11-01), Williams et al.
patent: 6361945 (2002-03-01), Becker et al.
patent: 7081527 (2006-07-01), Cunningham et al.
patent: 7585631 (2009-09-01), Cunningham et al.
patent: 2001/0053519 (2001-12-01), Fodor et al.
patent: 2002/0055116 (2002-05-01), Cunningham et al.
patent: 2002/0143111 (2002-10-01), Halverson et al.
patent: 2002/0193962 (2002-12-01), Yakhini et al.
patent: 2004/0072239 (2004-04-01), Renaud et al.
patent: 0652974 (1995-05-01), None
patent: WO 9402635 (1994-02-01), None
patent: WO 9404681 (1994-03-01), None
patent: WO 9634978 (1996-11-01), None
patent: WO 9640926 (1996-12-01), None
patent: WO 9702281 (1997-01-01), None
patent: WO 9703362 (1997-01-01), None
patent: WO 9708204 (1997-03-01), None
patent: WO97/35026 (1997-09-01), None
patent: WO 9742349 (1997-11-01), None
patent: WO 9804675 (1998-02-01), None
patent: WO 9806430 (1998-02-01), None
Awad-El-Kariem et al., “Detection and species identification ofCryptosporidiumoocysts using a system based on PCR and endonuclease restriction”, Parasitology, Jul. 1994, p. 19-22, vol. 109, Cambridge University Press, UK.
Balatbat et al., “Detection ofCryptosporidium parvumDNA in Human Feces by Nested PCR”, J. Clin. Microblol., Jul. 1996, p. 1769-1772, vol. 34, No. 7, American Society of Microbiology, US.
Carraway et al., “Genetic Markers DifferentiateC. parvumIsolates”, J. Eukaryot. Microbiol., Sep.-Oct. 1994, p. 26S, vol. 41, No. 5, Allen Press Inc., US.
Chrisp et al., “Similarities and differences between DNA ofCryptosporidium parvumandC. wrairidetected by the polymerase chain reaction”, Folia Parasitol., 1994, p. 97-100, vol. 41, No. 2, Czech Academy of Sciences, CZ.
Deere et al., “Rapid method for fluorescent in situ ribosomal rna labeling ofCryptosporidium parvum”, J. Appl. Microbiol., Nov. 1998, p. 807-818, vol. 85, No. 5, Blackwell Science Limited, UK.
Garcia et al., “Detection of Microsporidial Spores in Fecal Specimens from Patients Diagnosed with Cryptosporidiosis”, J. Clin. Microbiol., Jul. 1994, p. 1739-1741, vol. 32, No. 7, American Society of Microbiology, US.
Kilani et al., “Geographical Variation in 18S rRNA Gene Sequence ofCryptosporidium parvum”, Int. J. Parasitol., Apr. 1994, p. 303-306, vol. 24, No. 2, Elsevier Science Limited, UK.
Laxer at al., “DNA Sequences for the Specific Detection ofCryptosporidium parvumby the Polymerase Chain Reaction” Am. J. Trop. Med. Hyg., Dec. 1991, p. 688-694, vol. 45, No. 6, Allen Press Incorporated, US.
Lindquist et al., “Criteria for evaluation of proposed protozoan detection methods”, J. Microbiol. Methods, Jul. 1999, p. 33-43, vol. 37, No. 1, Elsevier Science Publishers BV, NE.
Long at al., “The Diagnosis of Old and New Gastrointestinal Parasites”, Clin. Lab. Med., Jun. 1995, p. 307-331, vol. 15, No. 2, W B Saunders Company, US.
Marsh at al., “Sequence Analysis and Comparison of Ribosomal DNA From Bovine Neospora to Similar Coccidial Parasites”, J. Parasitol., Aug. 1995, p. 530-535, vol. 81, No. 4, American Society of Parasitologists, US.
Morgan et al., “Differentiation Between Human and Animal Isolates ofCryptosporidlum parvumUsing rDNA Sequencing and Direct PCR Analysis”, J. Parasitol., Oct. 1997, p. 825-830, vol. 83, No. 5, American Society of Parasitologists, US.
Cai at al., “C. parvumribosomal RNA gene for 18S rRNA (pCPA911)”, Database NCBI ′Online!, Jun. 1993, Accession No. X64340.
Cai et al., “PCR cloning and nucleotide sequence determination of the 18S rRNA genes and internal transcribed spacer 1 of the protozoan parasitesCryptosporidium parvumandCryptosporidium muris”, Biochim Biophys Acta., Jul. 1992, 1131(3):317-20.
Deere et al., “Evaluation of fluorochromes for flow cytometric detection ofCryptosporidium parvumoocysts labelled by fluorescent in situ hybridization”, Lett Appl Microbiol., Dec. 1996, 27(6):352-6.
Deng et al., “Immunomagnetic Capture PCR to Detect ViableCryptosporidium parvumoocysts from Environmental Samples”, Appl Environ Microbiol., Aug. 1997, 63(8):3134-8.
Hallier-Soulier et al., “An immunomagnetic separation polymerase chain reaction assay for rapid and ultra-sensitive detection ofCryptosporidium parvumin drinking water”, FEMS Microbiol Lett., Jul. 1999, 176(2):285-9.
Laberge et al., “Detection ofCryptosporidium parvumin Raw Milk by PCR and Oligonucleotide Probe Hybridization”, Appl Environ Microbiol., Sep. 1996, 62(9):3259-64.
Lindquist, “Probes for the Specific Detection ofCryptosporidium parvum”, Wat Res., 1997, 31 (10):2668-71.
Nelson, Genbank Accession No. AA167899, Aug. 23, 2000.
Pieniazek at al., “Cryptosporidium parvum18S ribosomal RNA gene, complete”, Database NCBI ′Online!, Mar. 1996, Accession No. L16996.
Rochelle et al., “Comparison of primers and optimization of PCR conditions for detection ofCryptosporidium parvumandGiardi lambllain water”, Appl Environ Microbiol., Jan. 1997, 63(1):106-114, American Society for Microbiology, US.
Wang et al., “Electrochemical blosensor for detecting DNA sequences from the pathogenic protozoanCryptosporidium parvum”, Talanta, 1997, 44:2003-2010.
Morgan et al., “Phylogenetic Analysis ofCryptosporidiumisolates from Captive Reptiles Using 18S rDNA Sequence Data and Random Amplified Polymorphic DNA Analysis”, J. Parasitol., Jun. 1999, p. 525-530, vol. 85, No. 3, American Society of Parasitologists, US.
Rochelle et al., “Development of a Rapid Detection Procedure forCryptosporidium, Using in Vitro Cell Culture Combined with PCR”, J. Eukaryot. Microbiol., Sep.-Oct. 1996, p. 72S, vol. 43, No. 5, Allen Press, Inc., US.
Shepherd et al., “An Evaluation of Methods for the Simultaneous Detection ofCryptosporidiumOocysts and Giardia Cysts from Water”, Appl. Environ. Microbiol., Apr. 1996, p. 1317-1322, vol. 62, No. 4, American Society for Microbiology, US.
Singh, “Molecular Methods for Diagnosis and Epidemiological Studies of Parasitic Infections”, Int. J. Parasitol., Oct. 1997, p. 1135-1145, vol. 27, No. 10, Elsevier Science Limited, UK.
Slifko et al., “An In Vitro Method for Detecting InfectiousCryptosporidiumoocysts with Cell Culture”, Appl. Environ. Microbiol., Sep. 1997, p. 3669-3675, vol. 63, No. 9, American Society for Microbiology, US.
Vesey et al., “The use of a ribosomal RNA targeted oligonucleotide probe for fluorescent labelling of viableCryptosporidium parvumoocysts”, J. Appl. Microbiol., Sep. 1998, p. 429-440, vol. 85, No. 3, Bl
Cunningham Melissa M.
Stull Paul D.
Weisburg William G.
Cappellari Charles B.
Gen-Probe Incorporated
Whisenant Ethan C
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