Compositions and methods for detecting proteolytic activity

Details Compositions and methods for detecting proteolytic activity Compositions and methods for detecting proteolytic activity

2008-05-27

2008-05-27

Woitach, Joseph (Department: 1633)

Drug, bio-affecting and body treating compositions

In vivo diagnosis or in vivo testing

C424S009321, C424S009600, C424S093100, C424S094630, C435S069700, C435S320100, C435S325000, C435S455000

Reexamination Certificate

active

10452184

ABSTRACT:
The invention provides compositions and methods for non-invasive imaging of enzyme (e.g., protease) activity in cells, tissues and organs and entire bodies in vitro, in vivo and in situ. The invention provides a chimeric polypeptide having a bioluminescent or chemiluminescent polypeptide, or a heterologous kinase, and at least one silencing moiety, and a protease cleavage motif positioned between the first and second domains. The imaging can be by computer assisted tomography (CAT), magnetic resonance spectroscopy (MRS), magnetic resonance imaging (MRI), positron emission tomography (PET), single-photon emission computed tomography (SPECT), or bioluminescence imaging (BLI).

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