Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Reexamination Certificate
2005-05-31
2005-05-31
Winkler, Ulrike (Department: 1648)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
C424S093200, C424S093600, C424S208100, C424S233100, C435S006120, C435S008000, C435S014000, C435S069100, C435S070100, C435S173300, C435S207000, C435S235100, C435S242000, C435S320100, C435S325000, C435S372300, C435S455000, C514S04400A, C536S022100, C536S023400, C536S023720, C536S024100
Reexamination Certificate
active
06900010
ABSTRACT:
Methods are provided for screening natural and synthetic anti-HIV agents using recombinant cells that are rendered susceptible to productive infection of various strains, subtypes or clades of HIV from both laboratory and clinical isolates. The method comprises: taking a culture of recombinant cells in which at least one of the recombinant cells comprises a reporter sequence comprising a reporter gene whose expression is regulated by a protein specific to HIV, and a heterologous sequence which encodes CD4 and one or more additional cell surface receptors, wherein the heterologous sequence expresses CD4 and the one or more additional cell surface receptors at elevated levels as compared to the cell in the absence of expression by the heterologous sequence such that productive infection of the recombinant cell by HIV is achieved, which is defined by HIV viral replication and the infection of non-infected cells in the culture of the recombinant cells; contacting the cell culture with a sample containing HIV; adding a tester agent to the cell culture; and detecting a change in a level of expression of the reporter gene in the cells in the culture.
REFERENCES:
patent: 2 244 712 (1991-12-01), None
patent: WO 93/05185 (1993-03-01), None
patent: WO 00/43515 (2000-07-01), None
Jamieson, B.D. et al., “In Vivo Pathogenesis of a Human Immunodeficiency Virus Type 1 Reporter Virus”, Journal of Virology, Aug. 1998, vol. 72, No. 8, pp. 6520-6526.
Dorsky, D.I. et al., “Detection of HIV-1 Infection With a Green Fluorescent Protein Reporter System”, Journal of Immune Deficiency Syndromes, vol. 13, No. 4, 1996.
Lee, A.H. et al., “Generation of the Replication-Competent Human Immunodeficiency Virus Type 1 Which Expresses A Jellyfish Green Fluorescent Protein”, Biochemical and Biophysical Research Communications, vol. 233, pp. 288-292, 1997.
Koseki, S. et al., A Simple Assay System For Examination of the Inhibitory Potential In Vivo of Decoy RNA's, Ribozymes and other Drugs By Measuring the Tat-Mediated Transcription of a Fusion Gene Composed of the Long Terminal Repeat of HIV-1 and a Gene For Luciferase.
Gervaix, A. et al., “A New Reporter Cell Line To Monitor HIV Infection and Drug Susceptibility In Vitro”, Proc. Natl. Acad. Sci USA, vol. 94, pp. 4653-4658, Apr. 1997.
Pirounaki, Maria et al., “Rapid Phenotypic Drug Susceptibility Assay For HIV-1 With a CCR5 Expressing Indicator Cell Line”, Journal of Virological Methods 85 (2000), Ppages 151-161.
Mattias J. Schnell et al., Construction of a Novel Virus That Targets HIV-1-Infected Cells and Controls HIV-1 Infection, Cell, Sep. 5, 1997, vol. 90, pp. 849-857.
Yi-Fu Yang et al., IL-12 as well as IL-2 Upregulates CCR5 Expression on T Cell Receptor-Triggered Human CD4+ T Cells, Journal of Clinical Immunology, vol. 21, No. 2, pp. 116-125.
Adler Benjamin Aaron
MUSC Foundation For Research Development
Winkler Ulrike
LandOfFree
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