Compositions and methods for detecting cryptococcus neoformans

Details Compositions and methods for detecting cryptococcus neoformans Compositions and methods for detecting cryptococcus neoformans

2011-08-02

2011-08-02

Benzion, Gary (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

Reexamination Certificate

active

07989186

ABSTRACT:
Disclosed are oligonucleotides useful in methods for determining whether a sample containsCryptococcus neoformans, a causative agent for human cryptococcosis. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the fungal specific transcription factor gene inCryptococcus neoformans, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample containsCryptococcus neoformans. Real-time PCR and detection using florescence resonance energy transfer is disclosed.

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