Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-08-03
1996-08-13
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 435810, 436501, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, 935 88, C12Q 168, C12P 1934
Patent
active
055455247
ABSTRACT:
The present invention provides a rapid reproducible method for generating chromosome region-specific probes for diagnostic and research applications. The method of the present invention comprises microdissecting a chromosome of interest to produce a DNA fragment, treating the dissected DNA fragment with topoisomerase I, amplifying the treated dissected DNA fragment and labelling the amplified DNA. By utilizing the method of the present invention, region-specific probes for fluorescence in situ hybridization (FISH) from a single microdissected chromosome may be generated.
REFERENCES:
Guan et al., "Isolation of probes for a region of chromosome 6 deleted in malignant melanoma by chromosome microdissection" Proceedings of the American Association for Cancer Research, 82nd Annual Meeting, Houston, Texas (May 15-18, 1991) 32:302 (abstract no. 1795).
Taylor et al., "Visualisation of diagnostic tumour chromosome aberrations using fluorescence in situ hybridisation" Eleventh International Workshop On Human Gene Mapping, London, England, (Aug. 18-22, 1991), Cytogenet Cell Genet. (1991) 58: (1-4):2154, Abstract number 27372.
Cremer, T. et al., "Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome-region specific library probes." Hum. Genet. 80:235-246 (1988).
Bronstein, I. et al., "Rapid and Sensitive Detection of DNA in Southern Blots with Chemiluminescence." Bio Techniques 8:310-314 (1990).
Traver, C. N. et al., "Rapid Screening of a Human Genomic Library in Yeast Artificial Chromosomes for Single-Copy Sequences." PNAS, USA 86:5898-5902 (1989).
Hadano S. et al., "Laser Microdissection and Single Unique Primer Allow Generation of Regional Chromosome DNA Clones from a Single Human Chromosome." Genomics 11:364-373 (1991).
Lichter, P. et al., "Florescence in situ hybridization with Alu and L1 Polymerase Chain Reaction Probes for Rapid Characterization of Human Chromosomes in Hybrid Cell Lines." PNAS USA 87:6634-638 (1990).
Hampton et al., "Characterization and mapping of microdissected genomic clones from the adenomatous polyposis coli (APC) region" Genomics (1991) 11:247-251.
Johnson, "Molecular cloning of DNA from specific chromosomal regions by microdissection and sequence-indepepndent amplification of DNA" Genomics (1990) 6:243-251.
Kuo et al., "Detection of aneuploidy involving chromosomes 13, 18, or 21, by fluorescence in situ hybridization (FISH) to interphase and metaphase amniocytes" Am. J. Hum. Genet. (1991) 49:112-119.
Kuwano et al., "Detection of deletions and cryptic translocations in Miller-Dieker syndrome by in situ hybridization" Am. J. Hum. Genet. (1991) 49:707-714.
Lengauer et al., "Painting of defined chromosomal regions by in situ suppression hybridizaion of libraries from laser-microdissected chromosomes" Cytogenet. Cell. Genet. (1991) 56:27-30.
Lichter et al., "Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries" Hum. Genet. (1988) 80 224-234.
Pinkel et al., "Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4" Proc. Natl. Acad. Sci. USA (1988) 85:9138-9142.
Tkachuk et al., "Detection of bcr-abl fusion in chronic myelogenous leukemia by in situ hybridization" Science (1990) 250:559-562.
Trautmann et al., "Detection of APC region-specific signals by nonisotopic chromosomal in situ suppression (CISS)-hybridization using a microdissection library as a probe" Hum. Genet. (1991) 87:495-497.
Callen et al., "Chromosomal origin of small ring marker chromosomes in man: characterization by molecular genetics" Am. J. Hum. Genet. (1991) 48:769-782.
Meese et al., "Development and utilization of a somatic cell hybrid mapping panel to assign Notl linking probes to the long arm of human chromosome 6" Genomics (1992) 12: 542-548.
Fearon et al., "A genetic model for colorectal tumorigenesis" Cell (1990) 61:759-767.
Millikin et al., "Loss of heterozygosity for loci on the long arm of chromosome 6 in human malignant melanoma" Cancer Res. (1991) 51:5449-5453.
Mitelman et al., "Report of the committee on chromosome changes in neoplasia" Cytogenet. Cell Genet. (1991)00:000-000. A pre-print copy was previously submitted.
Nowell et al., "Chromosome translocations and oncogenes in human lymphoid tumors" Am. J. Clin. Pathol. (1990) 94:229-237.
Pathak et al., "Involvement of chromosome 6 in rearrangements in human malignant melanoma cell lines" Cytogenet. Cell. Genet. (1983) 36:573-579.
Rowley, "Molecular cytogenetics: Rosetta Stone for understanding cancer-twenty-ninth G.H.A. Clowes Memorial Award Lecture" Cancer Res. (1990) 50:3816-3825.
Trent et al., "Chromosome 6q involvement in human malignant melanoma" Cancer Genet. & Cytogenet. (1983) 9:177-180.
Trent et al., "Relation of cytogenetic abnormalities and clinical outcome in metastatic melanoma" New Eng. J. Med. (1990) 322:1508-1511.
Trent et al., "Tumorigenicity in human melanoma cell lines controlled by introduction of human chromosome 6" Science (1990) 247:568-571.
Fountain et al., "Physical mapping of a translocation breakpoint in neurofibromatosis" Science (1989) 244:1085-1087.
Warburton, "De novo balanced chromosome rearrangements and extra marker chromosomes identified at prenatal diagnosis: clinical significance and distribution of breakpoints" Am. J. Hum. Genet. (1991) 49:995-1013.
Haluska et al., "Oncogene activation by chromosome translocation in human malignancy" Ann. Rev. Genet. (1987) 21:321-345.
Weinberg, "A short guide to oncogenes and tumor-suppressor genes" J. NIH Res. (1991) 3:45-47.
Gelehrter et al., eds., Principles of Medical Genetics (1990) Williams & Wilkins, Baltimore, Maryland, pp. 156-189.
Hassold, "Chromosome abnormalities in human reproductive wastage" Trends in Genet. (Apr. 1986) pp. 105-110.
Wesley et al., "Cloning regions of the Drosophilagenome by microdissection of polytene chromosome DNA and PCR with nonspecific primer" Nucleic Acids Res. (1989) 18:599-603.
Cotter et al., "Gene mapping by microdissection and enzymatic amplification: heterogeneity in leukaemia associated breakpoints on chromosome 11" Genes, Chromosomes & Cancer (1991) 3:8-15.
Han et al., "Direct amplification of a single dissected chromosomal segment by polymerase chain reaction: a human brain sodium channel gene is on chromosome 2q22-q23" Proc. Natl. Acad. Sci. USA (1991) 88:335-339.
Ricciardi et al., "Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation" Proc. Natl. Acad. Sci. USA (1979) 76:4927-4931.
Hochgeschwender et al., "Construction and screening of a genomic library specific for mouse chromosome 16" Proc. Natl. Acad. Sci. USA (1989) 86:8482-8486.
Senger et al., "Microdissection of banded human chromosomes" Hum. Genet. (1990) 84:507-511.
Chehab et al., "Detection of specific DNA sequences by florescence amplification: a color complementation assay" Biochem. (1989) 86:9178-9182.
Ludecke et al., "Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification" Nature (1989) 338:348-350.
Meltzer Paul S.
Trent Jeffrey M.
Jones W. Gary
Marschel Ardin H.
The Regents of the University of Michigan
LandOfFree
Compositions and methods for chromosome region-specific probes does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Compositions and methods for chromosome region-specific probes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Compositions and methods for chromosome region-specific probes will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1047291