Compositions and methods for cDNA synthesis

Details Compositions and methods for cDNA synthesis Compositions and methods for cDNA synthesis

2003-09-03

2009-12-29

Chunduru, Suryaprabha (Department: 1637)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S006120, C435S091200, C530S350000

Reexamination Certificate

active

07638612

ABSTRACT:
Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided. Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.

REFERENCES:
patent: 5556771 (1996-09-01), Shen et al.
patent: 5561058 (1996-10-01), Gelfand et al.
patent: 5614387 (1997-03-01), Shen et al.
patent: 5693517 (1997-12-01), Gelfand et al.
patent: 5919900 (1999-07-01), Moyle et al.
patent: 6087489 (2000-07-01), Dean
patent: 6214542 (2001-04-01), Striker et al.
patent: 6300073 (2001-10-01), Zhao et al.
patent: 6416989 (2002-07-01), Abad et al.
patent: 6495350 (2002-12-01), Lee et al.
patent: 6521411 (2003-02-01), Hecker et al.
patent: 6664379 (2003-12-01), Kudlicki et al.
patent: 6767724 (2004-07-01), Lee et al.
patent: 7078208 (2006-07-01), Smith et al.
patent: 2003/0087237 (2003-05-01), Hong et al.
patent: 03 74 9359 (2006-11-01), None
Boehringer Mannheim 1997 Biochemicals Catalog, pp. 146-147, 150-153, and an instruction Manual from Titan RT-PCR system, Cat. No. 1855476.
Applied Biosystems—A guide to real-time PCR reagents and consumables—Reagents and consumables sequenc detection systems. pp. 1-11, 2000.
Ringel MD et al. Quantitative reverse transcription-polymerase chain reaction of circulating thyroglobulin mRNA for monitoring patients with thyroid carcinoma. J Clin Endocrinology & Metabolism, vol. 84, No. 11, pp. 4037-4042, 1999.
“Protocols and Applications Guide, Third Edition”, Promega Protocols and Applications Guide, 1996, pp. 181-189.
“Stratagene Catalog: RT-PCR Systems and Kits”, Stratagene Calalogue, 1999, pp. 154-155, XP002264374.
TaqMan Gold RT-PCR Kit Protocol, Applied Biosystems, Nov. 2006, pp. 1-78.
TaqMan One-Step RT-PCR Master Mix Reagents Kit, Applied Biosystems Product Bulletin, 2003, pp. 1-2.
TaqMan One-Step RT-PCR Master Mix Reagents Kit Protocol, Applied Biosystems, Nov. 2006, pp. 1-41.
TaqMan Preamp Master Mix (2X) Material Safety Data Sheet, Applied Biosystems, Feb. 16, 2009, pp. 1-6.
TaqMan Universal PCR Master Mix, No. UNG, Material Safety Data Sheet, Applied Biosystems, Mar. 5, 2009, pp. 1-6.
TaqMan Universal PCR Master Mix, No. UNG, Material Safety Data Sheet, Roche Diagnostics, 2006, pp. 1-11.

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