Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2005-09-20
2005-09-20
McElwain, Elizabeth (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C800S278000, C800S298000, C800S312000, C536S023100, C536S023600
Reexamination Certificate
active
06946589
ABSTRACT:
Methods and compositions for altering amino acid composition of a protein of interest are provided, particularly proteins whose three-dimensional structure is unknown. The method comprises creating interacting molecules to the native protein and selecting for engineered proteins which retain the native conformation by antibody binding. In this manner, the levels of essential amino acids in a protein can be increased yet the biological activity of the protein maintained. Also provided is an exemplary plant protein—Glycine maxvegetative storage protein (VSP)—in which methionine levels have been increased.
REFERENCES:
patent: 5288629 (1994-02-01), Berkner
patent: 5559223 (1996-09-01), Falco et al.
patent: 5589615 (1996-12-01), De Clercq et al.
patent: 6080913 (2000-06-01), Tarczynski
patent: WO 93/08682 (1993-05-01), None
patent: WO 94/16088 (1994-07-01), None
patent: WO 95/22625 (1995-08-01), None
patent: WO 95/31554 (1995-11-01), None
patent: WO 96/38563 (1996-12-01), None
patent: WO 97/20078 (1997-06-01), None
patent: WO 97/35023 (1997-09-01), None
Bendayan S. et al. The Journal of Histochemistry and Cytochemistry, 1995; vol. 43, No. 9; pp. 881-886.
Gordon-Kamm et al. The Plant Cell, Jul. 1990; vol. 2; pp. 603-608.
Jaynes J.M. Proceedings of Alltech's 10th Annual Symposium, Lexington KY May 1994, pp. 129-153.
Staswick P. The Plant Cell, Jan. 1990; vol. 2, pp. 1-6.
Dyer J.M. et al. Journal of Protein Chemistry, 1995; vol. 14, No. 8, pp. 665-678.
Altenbach et al., Enhancement of the Methionine Content of Seed Proteins by the Expression of a Chimeric Gene Encoding a Methionine-Rich Protein in Transgenic Plants, Plant Molecular Biology, 1989, pp. 513-522, vol. 13, Belgium.
Scott et al., Searching for Peptide Ligands with an Epitope Library, Science, 1990, pp. 386-390, vol. 249.
Staswick, P., Novel Regulation of Vegetative Storage Protein Genes, The Plant Cell, Jan. 1990, pp. 1-6, vol. 2.
Stemmer, W., Rapid Evolution of a Protein In Vitro By DNA Shuffling, Nature, Aug. 4, 1994, pp. 389-391, vol. 374.
Stemmer, W., DNA Shuffling by Random Fragmentation and Reassembly: In Vitro Recombination for Molecular Evolution, Proc. Natl. Acad. Sci. USA, Oct. 1994, pp 10747-10751, vol. 91.
Hongdi et al., A Phage Display System for Studying the Sequence Determinations of Protein Folding, Protein Science, 1995, pp. 1108-1117, vol. 4.
Marcellino et al., Modified 2S Albumins with Improved Tryptophan Content are Correctly Expressed in Transgenic Tobacco Plants, FEBS Letters, 1996, pp. 154-158, vol. 385.
Molvig et al., Enhanced Methionine Levels and Increased nutritive Value of Seeds of Transgenic Lupins (Lupinus angustifoliusL.) Expressing a Sunflower Seed Albumin Gene, Proc. Natl. Acad. Sci. USA, Aug. 1997, pp. 8393-8398, vol. 94, Agricultural Sciences.
Friguet et al., Immunochemical Analysis of Protein Conformation, Protein Structure a Practical Approach, pp. 287-310, IRL Press, Oxford University Press, MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Arnold, F.H., et al., “Optimizing Industrial Enzymes by Directed Evolution,”Adv. Biochem. Eng. Biotechnol.,1997, pp. 1-14, vol. 58.
Dyer, J.M., et al., “Extensive Modifications for Methionine Enhancement in the beta-Barrels Do Not Alter the Structural Stability of the Bean Seed Storage Protein Phaseolin,”J. Protein Chemistry,1995, pp. 665-678, vol. 14(8).
Goldberg, M.E., “Investigating Protein Conformation, Dynamics and Folding with Monoclonal Antibodies,”TIBS,1991, pp. 358-362, vol. 16(10).
Jaynes, J.M., “De Novo Designed Synthetic Plant Storage Proteins: Enhancing Protein Quality of Plants for Improved Human and Animal Nutrition,”Proceedings of Alltech's 10thAnnual Symposium,Lexington, KY, May 1994, pp. 129-153.
Lopes, M.A., “Approaches for Enhancing the Lysine Content of Maize Seed,”Biotechnol. Nutr. Proc. Int. Symp. 3rd, 1992, pp. 237-252.
Rao A. Gururaj
Sleister Heidi Major
Alston & Bird LLP
Kallis Russell
McElwain Elizabeth
Pioneer Hi-Bred International , Inc.
LandOfFree
Compositions and methods for altering amino acid content of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Compositions and methods for altering amino acid content of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Compositions and methods for altering amino acid content of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3399685