Compositions and elements containing triarylmethane leuco dyes a

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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422 56, 422 57, 422 60, 435805, 436135, 436169, 436170, C12Q 128, G01N 3350

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046703853

ABSTRACT:
A particular class of triarylmethane leuco dyes are useful in analytical compositions, elements and methods. These leuco dyes are triarylmethane compounds having an aromatic heterocyclic moiety attached to the central methane carbon and which, upon interaction with hydrogen peroxide, provide dyes having a maximum absorption at a wavelength equal to or greater than about 600 nm. Particularly useful leuco dyes have the structure: ##STR1## wherein R is a 5- to 15-membered aromatic heterocyclic moiety; and R' and R" are independently open chain or cyclic amines. These compounds are useful for providing detectable dyes in compositions and elements useful for detection of hydrogen peroxide or other analytes which react to produce hydrogen peroxide in aqueous liquids, e.g. biological fluids.

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Draper et al, J. Chromat., 216 (1981), pp. 413-416.
Mottola et al, Anal. Chem., 42(3), pp. 410-411 (1970).

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