Composition with modified pertussis toxin

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus

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530403, 530404, 530406, A61K 3910

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active

051659271

ABSTRACT:
A modified pertussis toxin suitable as a pertussis vaccine having an essentially unmodified B-oligomer and a catalytic subunit which is inactivated by treatment with polyphosphate compounds, sulfhydryl reductants and mild detergents followed by modification of the activated --SH groups to inhibit ADP-ribosylating activity.

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patent: 4699786 (1987-10-01), Lin et al.
patent: 4705686 (1987-11-01), Scott et al.
patent: 4762710 (1988-08-01), Sekura
patent: 4774086 (1988-09-01), Quentin-Millet et al.
patent: 4788058 (1988-11-01), Parton et al.
patent: 4996299 (1991-02-01), Ginnaga et al.
patent: 5032398 (1991-07-01), Kaslow
AR-Katada et al., "The A Promoter of Islet-Activating Protein, Pertussis Toxin, as an Active Peptide Catalyzing ADP-Ribosylation of a Membrane Protein", Archives of Biochemistry and Biophysics, vol. 224, No. 1, Jul. 1, pp. 290-298, 1983.
AS-Sekura et al., "Pertussis Toxin", The Journal of Biological Chemistry, vol. 258, No. 23, Dec. 10, pp. 14647-14651, 1983.
AT-Tamura et al., "Subunit Structure of Islet-Activating Protein, Pertussis Toxin, in Conformity with A-B Model", Journal of American Chemical Society, vol. 21, No. 22, 1982, pp. 5516-5522.
AR.sup.2 -Nogimori et al., "Chemical Modification of Islet-Activating Protein, Pertussis Toxin", Biochimica et Biophysica Acta, 801, (1984), pp. 220-231.
AS.sup.2 -Carroll et al., "Photoaffinity Labeling of Diphtheria Toxin Fragment A with NAD: Structure of the Photoproduct at Position 148", Proc. Natl. Acad. Sci. USA, 82, Nov. 1985, pp. 7237-7241.
AT.sup.2 -Leonard M. Hjelmeland, "A Nondenaturing Zwitterionic Detergent for Membrane Biochemistry: Design and Synthesis", Proc. Natl. Acad. Sci. USA, 77, No. 11, Nov. 1980, pp. 6368-6370.
AR.sup.3 -Means et al., "Chemical Modification of Proteins", Holden-Day, Inc. 1971, pp. 11-15, 19, 105-113, 149-159, 171.
AS.sup.3 -Lundblad et al., "Chemical Reagents for Protein Modification", vol. 1, CRC Press, Inc., Boca Raton, Fla., 1984, pp. 55-73 and 95-98.
AT.sup.3 -Duncan et al., "Chemical Inactivation of Pertussis Toxin", 1986 ASM Annual Meeting, Official Abstract, Mar. 23-28, 1986.
AR.sup.4 -Moss et al., Biochemistry, 25, 2720-5, 1986 (May 1986).

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