Composition useful for protein fragment complementation...

Details Composition useful for protein fragment complementation... Composition useful for protein fragment complementation...

2011-06-07

2011-06-07

Nashed, Nashaat T (Department: 1656)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

Reexamination Certificate

active

07955832

ABSTRACT:
The present invention describes an assay method comprising: (A) generating (1) at least a first fragment of a reporter molecule linked to a first interacting domain and at least a second fragment of a reporter molecule linked to a second interacting domain, or (2) nucleic acid molecules that code for (A)(1) and subsequently allowing said nucleic acid molecules to produce their coded products; then, (B) allowing interaction of said domains; and (C) detecting reconstituted reporter molecule activity, where said reporter molecule can react with a penicillin- or a cephalosporin-class substrate.

REFERENCES:
patent: 2004/0038317 (2004-02-01), Balint et al.
John et al., Two Pairs of Oppositely Charged Amino Acidfsr om Jun and Fos Confer Heterodimerization to GCN4 Leucine Zipper, The Journal of Biological Chemistry Inc. vol. 269 No. 23, Issue of Jun. 10, pp. 16247-16253, 1994.

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