Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1986-10-23
1989-02-07
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
422 57, 427 2, 435 26, 435805, C12Q 132, C12Q 126
Patent
active
048031585
ABSTRACT:
This invention relates to a composition for determining .beta.-hydroxybutyric acid, usable for doctors, nurses, and patients themselves simply and quickly without use of special instruments, and also relates to a method for preparing the said composition.
The composition of the invention oxidizes .beta.-hydroxybutyric acid under alkaline conditions and the presence of nicotineamide adenine dinucleotide (NAD) by .beta.-hydroxybutyric acid dehydrogenase, and the produced reduction-type NAD (NADH) reduces tetrazolium salt through an electron carrier to produce formazan developing color. The composition permits accurate measurement quickly and simply, and has excellent conservative stability, because it is a uniform solid-phase composition prepared by applying reagents necessary for the reaction of film impermeable to water together with a natural or synthesized film forming polymer. The invention further provides a method for preparing the said composition by applying, on a supporter impermeable to water, W/O emulsion which is formed by dispersing alkaline buffer, .beta.-hydroxybutyric acid dehydrogenase, NAD, electron carrier, and tetrazolium salt in an organic solvent insoluble in water together with a natural or synthesized film forming polymer.
REFERENCES:
patent: 3630957 (1971-12-01), Rey et al.
patent: 4254222 (1981-03-01), Owen
patent: 4273870 (1981-06-01), Mollering et al.
patent: 4351899 (1982-09-01), Owen
patent: 4356149 (1982-10-01), Kitajima et al.
patent: 4567024 (1986-01-01), Koyama et al.
Harano Yutaka
Shigeta Yukio
Takahaski Yoshinori
Yamada Shigeki
Kabushiki Kaisha Kyoto Daiichi Kagaku
Kabushiki Kaisha Sanwa Kagaku Kenkyusho
Saunders David A.
Warden Robert J.
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