Composition method for determining the presence of leukocyte cel

Details Composition method for determining the presence of leukocyte cel Composition method for determining the presence of leukocyte cel

1994-08-22

1995-11-07

Beisner, William H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 19, 435805, 436903, 560 1, 562589, C12Q 100, C12Q 144, C07C 5908

Patent

active

054647398

ABSTRACT:
A composition and method for determining the presence of leukocyte cells, esterase or protease in a test sample are disclosed. The composition contains a lactate ester having the structure ##STR1## wherein A is an alcohol blocking group, and wherein B-O- is a residue of compound B-OH and provides a detectable response when the lactate ester is hydrolyzed; and a buffer. One class of lactate ester has the structure ##STR2## wherein X is O, S or NR.sup.2, R is aryl or lower alkyl, R.sup.1 is hydrogen or lower alkyl, and R.sup.2 is hydrogen, lower alkyl or aryl.

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