Composition, kit, and method for assaying heparinano a method fo

Details Composition, kit, and method for assaying heparinano a method fo Composition, kit, and method for assaying heparinano a method fo

1988-08-26

1989-07-25

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

422 61, 436 69, 436 94, 436166, C12Q 156, G01N 2175, G01N 3386

Patent

active

048513369

ABSTRACT:
A composition, kit, and method for determining the concentration of heparin in a blood plasma sample and a method for making the composition. The method includes incubating a blood plasma sample with a known amount of Factor X.sub.a, combining the incubated product with an admixture of calcium chloride, brain phospholipids, and a buffered plasma fraction that has been produced by treating mammalian blood to substantially remove Factors II, VII, IX, and X while retaining Factor V and fibrinogen, and measuring the time it takes for clotting to occur after combining the incubated product with the admixture.

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