Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1996-12-06
1998-11-03
Weber, Jon P.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435183, C12N 952, C12N 900
Patent
active
058307419
ABSTRACT:
An enzyme composition for dissociating tissues is disclosed. The composition comprises purified collagenase I and collagenase II from Clostridium histolyticum in a mass ratio of about 0.3 to 0.6 and a neutral protease such that the ratio of total FITC casein units of activity to Wunsch units of activity in the composition is about 250:1 to about 600:1. The preferred neutral protease is thermolysin.
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Dwulet Francis E.
Smith Marilyn E.
Amick Marilyn L.
Boehringer Mannheim Corporation
Harris Brent A.
Weber Jon P.
Young D. Michael
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