Composition for the analysis of the alkaline phosphatase and met

Details Composition for the analysis of the alkaline phosphatase and met Composition for the analysis of the alkaline phosphatase and met

1980-09-25

1981-12-15

Wiseman, Thomas

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

252408, 424 7, C12Q 142

Patent

active

043060200

ABSTRACT:
A novel composition is disclosed for determining the activity of the alkaline phosphatase, which comprises a salt of p-nitrophenylphosphoric acid, a magnesium salt, sodium borate and 2-amino-2-hydroxymethyl-1,3-propanediol. The composition affords advantages over the known composition especially as regards sensitivity.

REFERENCES:
patent: 2359052 (1944-09-01), Scharer
patent: 3425912 (1969-02-01), Deutsch et al.
patent: 3466306 (1969-09-01), Babson
patent: 4132598 (1979-01-01), Modrovich
Ellis, "Quality Control of Serum Alkaline Phosphatase Assays. Project report and discussion of some factors affecting the assay", Chem. Absts., vol. 84, No. 17, p. 180 (1976), Abs. No. 117450x.
McComb et al., "Study of Optimum Buffer Conditions for Measuring Alkaline Phosphatase Activity in Human Serum", Clin. Chem., vol. 18, No. 2 (1972), pp. 97-104.

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